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small-RNA sequencing of sEV isolated from plasma of astronauts

Published by Open Science Data Repository | National Aeronautics and Space Administration | Metadata Last Checked: August 31, 2025 | Last Modified: 2025-08-21
We sought to determine whether the spaceflight environment can induce alterations in small extracellular vesicles (sEV) smallRNA content and their utility as biomarkers. Using small RNA sequencing (sRNAseq), we evaluated the impact of the spaceflight environment on sEV miRNA content in peripheral blood (PB) plasma of 14 astronauts, who flew STS missions between 1998-2001. Samples were collected at three-time points:10 days before the launch (L-10), the day of return (R-0), and three days post-landing (R+3).

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