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Species ID Project

Published by NMFS Office Of Sustainable Fisheries | National Oceanic and Atmospheric Administration, Department of Commerce | Metadata Last Checked: December 19, 2025 | Last Modified: 2024-02-29T00:00:00.000+00:00
Up to 10 individuals of a fin fish, shark, and crustacean species are collected and morphologically identified by Southeast Fisheries Science Center. Water-soluble sarcoplasmic proteins are extracted from the tissue; proteins are analyzed by microfluidic electrophoresis (Agilent Bioanalyzer 2100) to generate species-specific protein patterns. The protein patterns are entered into a database to determine which are the most abundant for each species. The proteins that are seen in all 10 individuals are transferred to the excel pattern matching library database that uses NSIL designed formulas to calculate the range of each protein (+/- 1.5 to 3%) and compare an unknown protein to pattern to those in the pattern matching library.

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