The Application of a Highly Purified Rat Leydig Cell Assay as a Complement to the H295R Steroidogenesis Assay to Evaluate Toxicant Induced Alterations in Testosterone Production
The greater dynamic range of testosterone production in a highly purified rat Leydig cell assay permitted the detection of chemical induced inhibition that was not detected by the high throughput screening format of the H295R steroidogenesis assay. This dataset is associated with ORD-022468 entered in STICS.
This dataset is associated with the following publication:
Botteri Principato, N., J. Suarez, S. Laws, and G. Klinefelter. The Use of Purified Rat Leydig Cells Complements the H295R Screen to Detect Chemical Induced Alterations in Testosterone Production. BIOLOGY OF REPRODUCTION. Society for the Study of Reproduction, 98(2): 239-249, (2018).
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"020:00"
]
|
| contactPoint |
{
"fn": "Gary Klinefelter",
"hasEmail": "mailto:klinefelter.gary@epa.gov"
}
|
| description | The greater dynamic range of testosterone production in a highly purified rat Leydig cell assay permitted the detection of chemical induced inhibition that was not detected by the high throughput screening format of the H295R steroidogenesis assay. This dataset is associated with ORD-022468 entered in STICS. This dataset is associated with the following publication: Botteri Principato, N., J. Suarez, S. Laws, and G. Klinefelter. The Use of Purified Rat Leydig Cells Complements the H295R Screen to Detect Chemical Induced Alterations in Testosterone Production. BIOLOGY OF REPRODUCTION. Society for the Study of Reproduction, 98(2): 239-249, (2018). |
| distribution |
[
{
"title": "manuscript figures 6-2-17.pdf",
"mediaType": "application/pdf",
"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1378085/manuscript%20figures%206-2-17.pdf"
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|
| identifier | https://doi.org/10.23719/1378085 |
| keyword |
[
"2nd Tier screen",
"Endocrine Disruptors",
"Leydig cell assay",
"cell culture",
"gonadal steroids",
"male infertility",
"steroidogenesis",
"testis",
"testosterone"
]
|
| license | https://pasteur.epa.gov/license/sciencehub-license.html |
| modified | 2017-06-16 |
| programCode |
[
"020:000"
]
|
| publisher |
{
"name": "U.S. EPA Office of Research and Development (ORD)",
"subOrganizationOf": {
"name": "U.S. Environmental Protection Agency",
"subOrganizationOf": {
"name": "U.S. Government"
}
}
}
|
| references |
[
"https://doi.org/10.1093/biolre/iox177"
]
|
| rights |
null
|
| title | The Application of a Highly Purified Rat Leydig Cell Assay as a Complement to the H295R Steroidogenesis Assay to Evaluate Toxicant Induced Alterations in Testosterone Production |
