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The Evolutionary Genetics of Mushroom-feeding Drosophila in Great Smoky Mountains National Park

Published by National Park Service | Department of the Interior | Metadata Last Checked: January 25, 2026 | Last Modified: 2019-10-30T00:00:00Z
Field Methods: Fly collections. Drosophila will be collected from forests during summer months (May to October) by sweep-netting or aspirating over naturally-occurring mushrooms. In some instances, boiled and thus killed commercial mushrooms (Agaricus bisporus) will be set out as baits. In addition, flies may be bred from field-collected mushrooms. Laboratory maintenance of fly cultures. In the laboratory, the culture medium consists of Instant Drosophila medium (Carolina Biological), a small piece of commercial mushroom, and a dental cotton roll, which serves as a pupation site. Cultures are maintained at 20C, 60%RH, and 14:10 light:dark schedule.

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