Transcriptional profiling of the right gastrocnemius muscle from mice flown on the RR-1 mission
In the NASA Rodent Research-1 (RR-1) validation study, ten 16-week-old female C57BL/6J mice were flown to the ISS for 37 days before euthanasia and subsequent dissection (Flight group). Due to crew time constraint, only two (out of ten) mice were dissected immediately after euthanasia to recover spleen and liver tissues on the ISS. The remaining eight animals were euthanized, then intact carcasses were placed in a pre-chilled cold stowage container and stored in the MELFI. There were respective cohorts of age-matched basal animals which were euthanized one day after launch as a baseline control (Basal control group) as well as age-matched ground control animals kept in an ISS Environmental Simulator at Kennedy Space Center (KSC) on a 4-day delay to mimic spaceflight conditions (Ground control group). In addition, the NASA Validation study also had a cohort of age-matched vivarium control animals that were housed in the vivarium cages and followed the same experimental timeline and process as the spaceflight animals (Vivarium control group). This dataset was generated only from Flight (n of 4) and Ground control (n of 8) animals that were euthanized and preserved intact on-orbit for subsequent dissection on the ground. Libraries were generated using a 3’ Tag-seq approach and sequenced at a depth of 40 M clusters (SE 93 bp).
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| description | In the NASA Rodent Research-1 (RR-1) validation study, ten 16-week-old female C57BL/6J mice were flown to the ISS for 37 days before euthanasia and subsequent dissection (Flight group). Due to crew time constraint, only two (out of ten) mice were dissected immediately after euthanasia to recover spleen and liver tissues on the ISS. The remaining eight animals were euthanized, then intact carcasses were placed in a pre-chilled cold stowage container and stored in the MELFI. There were respective cohorts of age-matched basal animals which were euthanized one day after launch as a baseline control (Basal control group) as well as age-matched ground control animals kept in an ISS Environmental Simulator at Kennedy Space Center (KSC) on a 4-day delay to mimic spaceflight conditions (Ground control group). In addition, the NASA Validation study also had a cohort of age-matched vivarium control animals that were housed in the vivarium cages and followed the same experimental timeline and process as the spaceflight animals (Vivarium control group). This dataset was generated only from Flight (n of 4) and Ground control (n of 8) animals that were euthanized and preserved intact on-orbit for subsequent dissection on the ground. Libraries were generated using a 3’ Tag-seq approach and sequenced at a depth of 40 M clusters (SE 93 bp). |
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| modified | 2025-08-21 |
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| title | Transcriptional profiling of the right gastrocnemius muscle from mice flown on the RR-1 mission |
