Transcriptional profiling of thymus from mice flown on the RR-9 mission
The objective of the Rodent Research-9 (RR-9) mission was to use mice to understand the molecular basis of phenomena that affect astronauts during long-duration spaceflight, particularly visual impairment, and joint tissue degradation. To this end, a flight group (FLT) of 10-week-old male C57BL/6J mice were launched from Kennedy Space Center (KSC) on 8/14/2017 and housed in Rodent Habitats on the ISS for 33 days before being returned alive to Earth. After splashdown in the Pacific Ocean, the animals were transported to Loma Linda University (LLU) for testing, euthanasia, and dissection on 9/18/2018. Ground Control (GC) studies were planned to commence at KSC approximately one-week after the conclusion of the flight experiments. However, all the GC mouse studies at KSC had to be cancelled due to Hurricane Irma and potential adverse effects on the animal housing facility. The GC studies were therefore rescheduled and begun in May 2018. The GC was euthanized and dissected 6/18/2018 - 6/20/2018. Because this resulted in a different cohort of mice being used for the GC controls as compared to the flight (FLT) groups, two cohort controls were included in the study. The first, Cohort Control 1 (CC_C1), was from the same cohort as the FLT animals and was sacrificed and dissected 4 days after the FLT group (9/22/2017). The second, Cohort Control 2 (CC_C2), was from the same cohort as the GC, and was sacrificed and dissected 2-8 days after the GC, (6/24/2018 - 6/26/2018). The CC_C1 and CC_C2 groups were housed in standard cages and fed standard chow in contrast to all other groups which received Rodent Foodbars. Upon dissection, thymus tissues were preserved in liquid nitrogen and stored at 80 C before RNA was extracted. Only the flight (FLT, n of 10) and Ground Control (GC, n of 10) samples were processed and analyzed in this study. Libraries were generated using a 3’ Tag-seq approach and sequenced at a targeted depth of 40 M clusters (SE 93 bp).
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| description | The objective of the Rodent Research-9 (RR-9) mission was to use mice to understand the molecular basis of phenomena that affect astronauts during long-duration spaceflight, particularly visual impairment, and joint tissue degradation. To this end, a flight group (FLT) of 10-week-old male C57BL/6J mice were launched from Kennedy Space Center (KSC) on 8/14/2017 and housed in Rodent Habitats on the ISS for 33 days before being returned alive to Earth. After splashdown in the Pacific Ocean, the animals were transported to Loma Linda University (LLU) for testing, euthanasia, and dissection on 9/18/2018. Ground Control (GC) studies were planned to commence at KSC approximately one-week after the conclusion of the flight experiments. However, all the GC mouse studies at KSC had to be cancelled due to Hurricane Irma and potential adverse effects on the animal housing facility. The GC studies were therefore rescheduled and begun in May 2018. The GC was euthanized and dissected 6/18/2018 - 6/20/2018. Because this resulted in a different cohort of mice being used for the GC controls as compared to the flight (FLT) groups, two cohort controls were included in the study. The first, Cohort Control 1 (CC_C1), was from the same cohort as the FLT animals and was sacrificed and dissected 4 days after the FLT group (9/22/2017). The second, Cohort Control 2 (CC_C2), was from the same cohort as the GC, and was sacrificed and dissected 2-8 days after the GC, (6/24/2018 - 6/26/2018). The CC_C1 and CC_C2 groups were housed in standard cages and fed standard chow in contrast to all other groups which received Rodent Foodbars. Upon dissection, thymus tissues were preserved in liquid nitrogen and stored at 80 C before RNA was extracted. Only the flight (FLT, n of 10) and Ground Control (GC, n of 10) samples were processed and analyzed in this study. Libraries were generated using a 3’ Tag-seq approach and sequenced at a targeted depth of 40 M clusters (SE 93 bp). |
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| title | Transcriptional profiling of thymus from mice flown on the RR-9 mission |
