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Vertical stratification of the water microbiome in an electric water heater tank

Published by U.S. EPA Office of Research and Development (ORD) | U.S. Environmental Protection Agency | Metadata Last Checked: August 02, 2025 | Last Modified: 2024-02-16
The raw sequence reads have been submitted to the NCBI Sequence Read Archive (SRA) under the BioProject PRJNA961987 with the following BioSample numbers: SAMN39596420, SAMN39596421, SAMN39596422, SAMN39596423, SAMN39596424, SAMN39596425, SAMN39596426, SAMN39596427, SAMN39596428, SAMN39596429, SAMN39596430, SAMN39596431, SAMN39596432, SAMN39596433, SAMN39596434, SAMN39596435, SAMN39596436, SAMN39596437, and SAMN39596438. A table (Table_diversity_indices.xlsx) contains rows as samples with ID, columns as distance from top to bottom (cm), sample volume (L), flushed volume (L), diversity indices (Chao and Shannon), and water temperature (°C) for each Set of samples. A table (Table_Legiolert.xlsx) contains rows as samples with ID, columns as distance from top to bottom (cm), sample volume (L), flushed volume (L), abundance of cultured Legionella pneumophila (MPN/100mL), and water temperature (°C) for each Set of samples. A table (Table_qPCR_L_pneumophila.xlsx) contains rows as samples with ID, columns as distance from top to bottom (cm), sample volume (L), flushed volume (L), copy number of L. pneumophila (GCN/100mL), and water temperature (°C) for each Set of samples. A table (Table_qPCR_Legionella.xlsx) contains rows as samples with ID, columns as distance from top to bottom (cm), sample volume (L), flushed volume (L), copy number of Legionella spp. (GCN/100mL), and water temperature (°C) for each Set of samples. A table (Table_qPCR_Mycobacterium.xlsx) contains rows as samples with ID, columns as distance from top to bottom (cm), sample volume (L), flushed volume (L), copy number of Mycobacterium spp. (GCN/100mL), and water temperature (°C) for each Set of samples. A table (Table_qPCR_V_vermiformis.xlsx) contains rows as samples with ID, columns as distance from top to bottom (cm), sample volume (L), flushed volume (L), copy number of V. vermiformis (GCN/100mL), and water temperature (°C) for each Set of samples. An abundance matrix (Table_taxonomy.xlsx) contains rows as taxonomic lineage, columns as samples, and entries representing the abundance of each lineage as a number of reads obtained for each Set of samples. A table (Table_water_temperature.xlsx) contains rows as water samples with ID, columns as temperature (°C and °F) measured from the top, bottom and drain of the water tank for each Set of samples. This dataset is associated with the following publication: Gomez-Alvarez, V., H. Ryu, M. McNeely, C. Muhlen, D. Williams, D. Lytle, and L. Boczek. Vertical Stratification of the Water Microbiome in an Electric Water Heater Tank: implications for premise plumbing opportunistic pathogens. JOURNAL OF WATER AND HEALTH. IWA Publishing, London, UK, 22(12): 2346–2357, (2024).

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Complete Metadata
accessLevel public
bureauCode 
                                            [
  "020:00"
]
contactPoint 
                                            {
  "fn": "Vicente Gomez-Alvarez",
  "hasEmail": "mailto:gomez-alvarez.vicente@epa.gov"
}
description The raw sequence reads have been submitted to the NCBI Sequence Read Archive (SRA) under the BioProject PRJNA961987 with the following BioSample numbers: SAMN39596420, SAMN39596421, SAMN39596422, SAMN39596423, SAMN39596424, SAMN39596425, SAMN39596426, SAMN39596427, SAMN39596428, SAMN39596429, SAMN39596430, SAMN39596431, SAMN39596432, SAMN39596433, SAMN39596434, SAMN39596435, SAMN39596436, SAMN39596437, and SAMN39596438. A table (Table_diversity_indices.xlsx) contains rows as samples with ID, columns as distance from top to bottom (cm), sample volume (L), flushed volume (L), diversity indices (Chao and Shannon), and water temperature (°C) for each Set of samples. A table (Table_Legiolert.xlsx) contains rows as samples with ID, columns as distance from top to bottom (cm), sample volume (L), flushed volume (L), abundance of cultured Legionella pneumophila (MPN/100mL), and water temperature (°C) for each Set of samples. A table (Table_qPCR_L_pneumophila.xlsx) contains rows as samples with ID, columns as distance from top to bottom (cm), sample volume (L), flushed volume (L), copy number of L. pneumophila (GCN/100mL), and water temperature (°C) for each Set of samples. A table (Table_qPCR_Legionella.xlsx) contains rows as samples with ID, columns as distance from top to bottom (cm), sample volume (L), flushed volume (L), copy number of Legionella spp. (GCN/100mL), and water temperature (°C) for each Set of samples. A table (Table_qPCR_Mycobacterium.xlsx) contains rows as samples with ID, columns as distance from top to bottom (cm), sample volume (L), flushed volume (L), copy number of Mycobacterium spp. (GCN/100mL), and water temperature (°C) for each Set of samples. A table (Table_qPCR_V_vermiformis.xlsx) contains rows as samples with ID, columns as distance from top to bottom (cm), sample volume (L), flushed volume (L), copy number of V. vermiformis (GCN/100mL), and water temperature (°C) for each Set of samples. An abundance matrix (Table_taxonomy.xlsx) contains rows as taxonomic lineage, columns as samples, and entries representing the abundance of each lineage as a number of reads obtained for each Set of samples. A table (Table_water_temperature.xlsx) contains rows as water samples with ID, columns as temperature (°C and °F) measured from the top, bottom and drain of the water tank for each Set of samples. This dataset is associated with the following publication: Gomez-Alvarez, V., H. Ryu, M. McNeely, C. Muhlen, D. Williams, D. Lytle, and L. Boczek. Vertical Stratification of the Water Microbiome in an Electric Water Heater Tank: implications for premise plumbing opportunistic pathogens. JOURNAL OF WATER AND HEALTH. IWA Publishing, London, UK, 22(12): 2346–2357, (2024).
distribution 
                                            [
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    "title": "https://www.ncbi.nlm.nih.gov/biosample/SAMN39596420",
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    "accessURL": "https://www.ncbi.nlm.nih.gov/biosample/SAMN39596421"
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    "accessURL": "https://www.ncbi.nlm.nih.gov/biosample/SAMN39596422"
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    "accessURL": "https://www.ncbi.nlm.nih.gov/biosample/SAMN39596423"
  },
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    "accessURL": "https://www.ncbi.nlm.nih.gov/biosample/SAMN39596424"
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    "accessURL": "https://www.ncbi.nlm.nih.gov/biosample/SAMN39596427"
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  {
    "title": "https://www.ncbi.nlm.nih.gov/biosample/SAMN39596428",
    "accessURL": "https://www.ncbi.nlm.nih.gov/biosample/SAMN39596428"
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    "accessURL": "https://www.ncbi.nlm.nih.gov/biosample/SAMN39596429"
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    "accessURL": "https://www.ncbi.nlm.nih.gov/biosample/SAMN39596436"
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    "accessURL": "https://www.ncbi.nlm.nih.gov/biosample/SAMN39596437"
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    "accessURL": "https://www.ncbi.nlm.nih.gov/biosample/SAMN39596438"
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  {
    "title": "Table_Legiolert.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1530693/Table_Legiolert.xlsx"
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  {
    "title": "Table_diversity_indices.xlsx",
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    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1530693/Table_diversity_indices.xlsx"
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  {
    "title": "Table_qPCR_L_pneumophila.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1530693/Table_qPCR_L_pneumophila.xlsx"
  },
  {
    "title": "Table_qPCR_Legionella.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1530693/Table_qPCR_Legionella.xlsx"
  },
  {
    "title": "Table_qPCR_Mycobacterium.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1530693/Table_qPCR_Mycobacterium.xlsx"
  },
  {
    "title": "Table_qPCR_V_vermiformis .xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1530693/Table_qPCR_V_vermiformis%20.xlsx"
  },
  {
    "title": "Table_taxonomy.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1530693/Table_taxonomy.xlsx"
  },
  {
    "title": "Table_water_temperature.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1530693/Table_water_temperature.xlsx"
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]
identifier https://doi.org/10.23719/1530693
keyword 
                                            [
  "Legionella pneumophila",
  "Premise Plumbing",
  "hot water tank",
  "microbial communities",
  "opportunistic pathogens"
]
license https://pasteur.epa.gov/license/sciencehub-license.html
modified 2024-02-16
programCode 
                                            [
  "020:000"
]
publisher 
                                            {
  "name": "U.S. EPA Office of Research and Development (ORD)",
  "subOrganizationOf": {
    "name": "U.S. Environmental Protection Agency",
    "subOrganizationOf": {
      "name": "U.S. Government"
    }
  }
}
references 
                                            [
  "https://doi.org/10.2166/wh.2024.265"
]
rights 
                                            null
title Vertical stratification of the water microbiome in an electric water heater tank

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