Zebrafish larvae submitted to simulated microgravity on a clinostat (CLINO) at 5dpf for one day
The general objective of the study was to determine modulation of gene expression by environmental factors specifically simulation of microgravity with a special emphasis on bone formation. For this reason the specific period of treatment was chosen between 5-6 days post-fertilization (dpf) when bone formation and calcification are taking place. Zebrafish larvae were placed at 5 dpf into a clinostat (CLINO) for 24 hours which was shown to simulate microgravity by the specific rotational movements it generates. We show that CLINO exposure causes a clear decrease of bone formation as illustrated by cranial skeleton staining of the bone matrix by Alizarin Red by morphometric analysis of the resulting images. Thus a whole genome micro-array experiment was conducted to identify genes that may be involved in the observed effect on bone formation.
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | irregular |
| bureauCode |
[
"026:00"
]
|
| contactPoint |
{
"fn": "GeneLab Outreach",
"@type": "vcard:Contact",
"hasEmail": "mailto:genelab-outreach@lists.nasa.gov"
}
|
| description | The general objective of the study was to determine modulation of gene expression by environmental factors specifically simulation of microgravity with a special emphasis on bone formation. For this reason the specific period of treatment was chosen between 5-6 days post-fertilization (dpf) when bone formation and calcification are taking place. Zebrafish larvae were placed at 5 dpf into a clinostat (CLINO) for 24 hours which was shown to simulate microgravity by the specific rotational movements it generates. We show that CLINO exposure causes a clear decrease of bone formation as illustrated by cranial skeleton staining of the bone matrix by Alizarin Red by morphometric analysis of the resulting images. Thus a whole genome micro-array experiment was conducted to identify genes that may be involved in the observed effect on bone formation. |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "Zebrafish larvae submitted to simulated microgravity on a clinostat (CLINO) at 5dpf for one day",
"format": "HTML",
"mediaType": "text/html",
"description": "GeneLab Study Page",
"downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-76"
}
]
|
| identifier | nasa_genelab_GLDS-76_ps96-ni2k |
| issued | 2018-06-26 |
| keyword |
[
"array-scanning-and-feature-extraction",
"growth-protocol",
"labeling",
"normalization-data-transformation",
"nucleic-acid-hybridization",
"p-mtab-48667",
"p-mtab-48668",
"p-mtab-48669",
"p-mtab-48670",
"p-mtab-48671",
"p-mtab-48672",
"p-mtab-48673",
"rna-extraction",
"stress",
"treatment-protocol"
]
|
| landingPage | https://data.nasa.gov/dataset/zebrafish-larvae-submitted-to-simulated-microgravity-on-a-clinostat-clino-at-5dpf-for-one- |
| modified | 2025-04-23 |
| programCode |
[
"026:005"
]
|
| publisher |
{
"name": "National Aeronautics and Space Administration",
"@type": "org:Organization"
}
|
| theme |
[
"Earth Science"
]
|
| title | Zebrafish larvae submitted to simulated microgravity on a clinostat (CLINO) at 5dpf for one day |
