Found 125 datasets matching "dna-polymerase".
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Background The thymidine kinase (tk) mutagenesis assay is often utilized to determine the frequency of herpes simplex virus (HSV) replication-mediated mutations. Using this assay,...
Search relevance: 200.45 | Views last month: 0 -
This data shows the quantification cycle at which fluorescence signals crossed a threshold fluorescence for samples analyzed as part of controlled experiments to determine whether contaminating...
Search relevance: 139.25 | Views last month: 0 -
This data set represents environmental DNA (eDNA) data obtained from water samples collected in the Sacramento River watershed and in the Central Valley, CA, USA. Specifically, Metabarcoding and...
Search relevance: 100.44 | Views last month: 13 -
Background In a variant of the standard PCR reaction termed bridging, or jumping, PCR the primer-bound sequences are originally on separate template molecules. Bridging can occur if, and...
Search relevance: 95.16 | Views last month: 0 -
These are the data from a laboratory experiment in which DNA content was removed from blood smears and extracted. The blood smears were either stained with Giemsa-stain or remained un-stained....
Search relevance: 82.67 | Views last month: 0 -
Water samples from the Platte River were collected and analyzed for environmental DNA (eDNA). The U.S. Geological Survey (USGS) Nebraska Water Science Center (NEWSC) collected samples monthly from...
Search relevance: 78.46 | Views last month: 0 -
This disclosure describes three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of the bacterial RecA and related proteins, to...
Search relevance: 76.04 | Views last month: 0 -
The data contain the raw quantitative polymerase chain reaction (qPCR) results after pre-amplification by polymerase chain reaction for all Ceracystis lukuohia and huliohia testing of...
Search relevance: 69.63 | Views last month: 1 -
These data include the quantitative Polymerase Chain Reaction (qPCR) Ceratocystis lukuohia and C. huliohia DNA confirmations of viable subcultures. These data were used to determine the accuracy...
Search relevance: 64.85 | Views last month: 0 -
These data contain the raw quantitative polymerase chain reaction (qPCR) results for all Ceratocystis lukuohia and huliohia testing of environmental DNA (eDNA) collected in the wind column using...
Search relevance: 63.90 | Views last month: 1 -
Background The DNA-dependent RNA polymerase from T7 bacteriophage (T7 RNAP) has been extensively characterized, and like other phage RNA polymerases it is highly specific for its...
Search relevance: 62.96 | Views last month: 0 -
The dataset provides the raw data in support of Tables 1-4 of the paper and include: colony forming unit (CFU) data for each of three replicates of sand, loam, and clay soils; results for the...
Search relevance: 59.62 | Views last month: 0 -
This data release documents specificity of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia, western mosquito fish and guppies. These assays...
Search relevance: 58.84 | Views last month: 0 -
This data set includes: (1) allele sizes of 11 previously published microsatellites (Kenkel and others, 2013; Shearer and Coffroth, 2004) for 39 individuals of mustard hill coral, Porites...
Search relevance: 58.50 | Views last month: 0 -
This data release includes metadata and tabular datasets that document (1) Austropuccina, Ceratocystis and Myrtaceae qPCR (quantitative polymerase chain reaction) DNA detections in Passive...
Search relevance: 57.84 | Views last month: 0 -
Data included are from a series of field sample collections from Lakes Michigan and Huron, and laboratory mesocosms targeting the round goby fish (Neogobius melanostomus). The round goby is a...
Search relevance: 57.55 | Views last month: 0 -
A modeling and analysis tool, “artemis,” was developed to analyze quantitative Polymerase Chain Reaction data from environmental DNA studies. Artemis models were fit to data from two field...
Search relevance: 57.08 | Views last month: 0 -
This data release documents sensitivity of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia, western mosquito fish and guppies. These assays...
Search relevance: 56.93 | Views last month: 0 -
These data contain the documentation of culturing results of Ceratocystis lukuohia and Ceratocystis huliohia from ambrosia beetle (Coleoptera: Curculionidae) bodies. Beetles were collected from...
Search relevance: 56.70 | Views last month: 0 -
Scientists at NOAA's Northeast Fisheries Science Center (NEFSC) are using environmental DNA (eDNA) to identify fish communities and monitor ecosystems by collecting a water sample and analyzing...
Search relevance: 55.68 | Views last month: 1
