Found 16 datasets matching "rna-amplification".
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Background High density cDNA microarray technology provides a powerful tool to survey the activity of thousands of genes in normal and diseased cells, which helps us both to understand...
Search relevance: 126.53 | Views last month: 0 -
A combination of linear RNA amplification and DNA microarray hybridization has allowed the determination of expression profiles of individual imaginal discs and larval tissues and the...
Search relevance: 126.24 | Views last month: 0 -
Irradiated cell lines exposed to 1-10 Gy 2 Lymphoblastoid cell lines (GM15510 and GM15036) irradiated 1 2.5 5 7.5 10 Gy RNA is isolated and labeled using a T7 amplification Arcturus kit for...
Search relevance: 66.38 | Views last month: 0 -
Irradiated cell lines exposed to 1-10 cGy 2 Lymphoblastoid cell lines (GM15510 and GM15036) irradiated 1 2.5 5 7.5 10 cGy RNA is isolated and labeled using a T7 amplification Arcturus kit for...
Search relevance: 64.91 | Views last month: 0 -
Background The DNA-dependent RNA polymerase from T7 bacteriophage (T7 RNAP) has been extensively characterized, and like other phage RNA polymerases it is highly specific for its...
Search relevance: 60.14 | Views last month: 0 -
Background Human herpesvirus-8 (HHV-8) is linked to the pathogenesis of Kaposi's sarcoma (KS), and the HHV-8 DNA load in peripheral blood mononuclear cells (PBMC) is associated with the...
Search relevance: 52.98 | Views last month: 0 -
Our aim was to identify specifically expressed genes using RNA arbitrarily primed (RAP)-polymerase chain reaction (PCR) for differential display in patients with rheumatoid arthritis (RA). In RA,...
Search relevance: 49.62 | Views last month: 0 -
Age plays a major role in tumor incidence and is an important consideration when modeling the carcinogenesis process or estimating cancer risks. Epidemiological data show that from adolescence...
Search relevance: 47.55 | Views last month: 0 -
Proton irradiation is touted for its improved tumor targeting due to the physical advantages of ion beams for radiotherapy. Recent studies from our laboratory have shown that in addition to...
Search relevance: 47.53 | Views last month: 0 -
Background We describe an alternative method to determine mRNA half-life (t1/2) based on the Real-Time RT-PCR procedure. This approach was evaluated by using the β-actin gene as a...
Search relevance: 47.25 | Views last month: 0 -
Proton irradiation is touted for its improved tumor targeting due to the physical advantages of ion beams for radiotherapy. Recent studies from our laboratory have shown that in addition to...
Search relevance: 47.01 | Views last month: 0 -
Taxonomic composition of environmental DNA acquired by filtration from the St. Regis River, New York
Environmental DNA (eDNA) surveys have become important tools for monitoring aquatic biodiversity. Barcode sequencing of eDNA generates community profiles that, while potentially biased in both...
Search relevance: 43.09 | Views last month: 1 -
Background Vacuolar type H+-ATPases play a critical role in the maintenance of vacuolar homeostasis in plant cells. V-ATPases are also involved in plants' defense against environmental...
Search relevance: 40.26 | Views last month: 0 -
Groundwater was collected by dead-end ultrafiltration and small-volume grab sampling from 138 wells in southwest Wisconsin across Grant, Iowa, and Lafayette Counties. Samples were collected to...
Search relevance: 33.06 | Views last month: 1 -
There is an increasing emphasis on effects-based monitoring to document responses associated with exposure to complex mixtures of chemicals, climate change, pathogens, parasites and other...
Search relevance: 31.99 | Views last month: 0 -
Groundwater samples were collected in June 2018 from a background (reference) well located 200 m upgradient from the source and five wells along a flowline in the plume at 39, 68, 102, 125, and...
Search relevance: 26.13 | Views last month: 0