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ATP BINDING CASSETTE SUB-FAMILY MEMBER 2 (ABCG2) AND XENOBIOTIC EXPOSURE DURING EARLY MOUSE EMBRYONIC STEM CELL DIFFERENTIATION

Published by U.S. EPA Office of Research and Development (ORD) | U.S. Environmental Protection Agency | Metadata Last Checked: August 02, 2025 | Last Modified: 2017-05-10
ATP binding cassette sub-family member 2 (ABCG2) is a well-defined efflux transporter found in various tissues. The role of ABCG2 during early embryonic development, however, is not established. Previous work suggested an association exists between xenobiotics that regulate Abcg2 transcription and differentiation of mouse embryonic stem cells (mESC), a relationship potentially related to redox homeostasis. ABCG2 was found to serve protective role in mESC as a xenobiotic transporter. The role of ABCG2 in regulating redox status, however, was unclear. The hypothesis that ABCG2 plays a fundamental role during mESC differentiation or that regulation of the receptor by xenobiotics may be associated with altered mESC differentiation could not be supported. This dataset is associated with the following publication: Rosen, M., S. Jeffay, H. Nichols, M. Hoopes, and S. Hunter. ATP Binding Cassette Sub-Family Member 2 (ABCG2) And Xenobiotic Exposure During Early Mouse Embryonic Stem Cell Differentiation. Birth Defects Research. John Wiley & Sons, Inc., Hoboken, NJ, USA, 110(1): 35-47, (2017).

Resources

11 resources available

  • manuscript figure 1.marker genes across days.xlsx

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  • manuscript figure 2.inhibitor exposure and normalized PhA FL on day 4.xlsx

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  • manuscript figure 3a.RedDot1 fluorescence on culture day 4 in mESC treated with topoisomerase inhibitors.xlsx

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  • manuscript figure 3b. Fold change in mESC on culture day 4 treated with topoisomerase inhibitors.docx

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  • manuscript figure 4a.PhA FL Day1 v Day4.xlsx

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  • manuscript figure 4b.Wes-simple western ABCG2 prot expression two reps.xlsx

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  • manuscript figure 5a b.cell number and gene expression on culture day 4 n K0143 treated mESC.xlsx

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  • manuscript figure 6a.RedDot1 FL in ToxCast treated mESC on culture day9.xlsx

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  • manuscript figure 6b. Fold change in ToxCast treated mESC on culture day 9.docx

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  • manuscript figure 7a. RedDot1 Fl in TBHP and Paraquat treated mESC on culture day4.xlsx

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  • manuscript figure 7b.Fold change in mESC on culture day 4 treated with tert-Butyl hydroperoxide and paraquat.docx

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Complete Metadata
accessLevel public
bureauCode 
                                            [
  "020:00"
]
contactPoint 
                                            {
  "fn": "Edward Hunter",
  "hasEmail": "mailto:hunter.sid@epa.gov"
}
description ATP binding cassette sub-family member 2 (ABCG2) is a well-defined efflux transporter found in various tissues. The role of ABCG2 during early embryonic development, however, is not established. Previous work suggested an association exists between xenobiotics that regulate Abcg2 transcription and differentiation of mouse embryonic stem cells (mESC), a relationship potentially related to redox homeostasis. ABCG2 was found to serve protective role in mESC as a xenobiotic transporter. The role of ABCG2 in regulating redox status, however, was unclear. The hypothesis that ABCG2 plays a fundamental role during mESC differentiation or that regulation of the receptor by xenobiotics may be associated with altered mESC differentiation could not be supported. This dataset is associated with the following publication: Rosen, M., S. Jeffay, H. Nichols, M. Hoopes, and S. Hunter. ATP Binding Cassette Sub-Family Member 2 (ABCG2) And Xenobiotic Exposure During Early Mouse Embryonic Stem Cell Differentiation. Birth Defects Research. John Wiley & Sons, Inc., Hoboken, NJ, USA, 110(1): 35-47, (2017).
distribution 
                                            [
  {
    "title": "manuscript figure 1.marker genes across days.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1377399/manuscript%20figure%201.marker%20genes%20across%20days.xlsx"
  },
  {
    "title": "manuscript figure 2.inhibitor exposure and normalized PhA FL on day 4.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1377399/manuscript%20figure%202.inhibitor%20exposure%20and%20normalized%20PhA%20FL%20on%20day%204.xlsx"
  },
  {
    "title": "manuscript figure 3a.RedDot1 fluorescence on culture day 4 in mESC treated with topoisomerase inhibitors.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1377399/manuscript%20figure%203a.RedDot1%20fluorescence%20on%20culture%20day%204%20in%20mESC%20treated%20with%20topoisomerase%20inhibitors.xlsx"
  },
  {
    "title": "manuscript figure 3b. Fold change in mESC on culture day 4 treated with topoisomerase inhibitors.docx",
    "mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1377399/manuscript%20figure%203b.%20Fold%20change%20in%20mESC%20on%20culture%20day%204%20treated%20with%20topoisomerase%20inhibitors.docx"
  },
  {
    "title": "manuscript figure 4a.PhA FL Day1 v Day4.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1377399/manuscript%20figure%204a.PhA%20FL%20Day1%20v%20Day4.xlsx"
  },
  {
    "title": "manuscript figure 4b.Wes-simple western ABCG2 prot expression two reps.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1377399/manuscript%20figure%204b.Wes-simple%20western%20ABCG2%20prot%20expression%20two%20reps.xlsx"
  },
  {
    "title": "manuscript figure 5a b.cell number and gene expression on culture day 4 n K0143 treated mESC.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1377399/manuscript%20figure%205a%20b.cell%20number%20and%20gene%20expression%20on%20culture%20day%204%20n%20K0143%20treated%20mESC.xlsx"
  },
  {
    "title": "manuscript figure 6a.RedDot1 FL in ToxCast treated mESC on culture day9.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1377399/manuscript%20figure%206a.RedDot1%20FL%20in%20ToxCast%20treated%20mESC%20on%20culture%20day9.xlsx"
  },
  {
    "title": "manuscript figure 6b. Fold change in ToxCast treated mESC on culture day 9.docx",
    "mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1377399/manuscript%20figure%206b.%20Fold%20change%20in%20ToxCast%20treated%20mESC%20on%20culture%20day%209.docx"
  },
  {
    "title": "manuscript figure 7a. RedDot1 Fl in TBHP and Paraquat treated mESC on culture day4.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1377399/manuscript%20figure%207a.%20RedDot1%20Fl%20in%20TBHP%20and%20Paraquat%20treated%20mESC%20on%20culture%20day4.xlsx"
  },
  {
    "title": "manuscript figure 7b.Fold change in mESC on culture day 4 treated with tert-Butyl hydroperoxide and paraquat.docx",
    "mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1377399/manuscript%20figure%207b.Fold%20change%20in%20mESC%20on%20culture%20day%204%20treated%20with%20tert-Butyl%20hydroperoxide%20and%20paraquat.docx"
  }
]
identifier https://doi.org/10.23719/1377399
keyword 
                                            [
  "ABCG2",
  "ToxCast",
  "differentiation",
  "mouse embryonic stem cell",
  "redox stress",
  "xenobiotics"
]
license https://pasteur.epa.gov/license/sciencehub-license.html
modified 2017-05-10
programCode 
                                            [
  "020:095"
]
publisher 
                                            {
  "name": "U.S. EPA Office of Research and Development (ORD)",
  "subOrganizationOf": {
    "name": "U.S. Environmental Protection Agency",
    "subOrganizationOf": {
      "name": "U.S. Government"
    }
  }
}
references 
                                            [
  "https://doi.org/10.1002/bdr2.1114"
]
rights 
                                            null
title ATP BINDING CASSETTE SUB-FAMILY MEMBER 2 (ABCG2) AND XENOBIOTIC EXPOSURE DURING EARLY MOUSE EMBRYONIC STEM CELL DIFFERENTIATION

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