Black et al_human rat and trout CLint_ScienceHub entry
This dataset provides measured in vitro intrinsic clearance rates for 54 chemicals tested using isolated hepatocytes from humans, rats, and rainbow trout. The test chemicals were selected to provide broad coverage across the industrial and pesticidal chemical space while also prioritizing chemicals of interest to EPA’s Endocrine Disruptor Screening Program (EDSP). A data evaluation framework was developed to identify results suitable for rate reporting. Acceptable results were then used to evaluate the chemical domain of applicability of the applied methods, the influence of starting substrate concentration on measured rates of intrinsic clearance, and differences in metabolic activity among species. These findings provide data for chemicals of specific interest to the EDSP. More importantly, the results provide critical guidance on future use of in vitro biotransformation assays to support high-throughput chemical risks assessments.
This dataset is associated with the following publication:
Black, S., J. Nichols, K. Fay, S. Matten, and S. Lynn. Evaluation and comparison of in vitro intrinsic hepatic clearance rates measured using cryopreserved hepatocytes from humans, rats, and rainbow trout. TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 457: 152819, (2021).
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"020:00"
]
|
| contactPoint |
{
"fn": "John Nichols",
"hasEmail": "mailto:nichols.john@epa.gov"
}
|
| description | This dataset provides measured in vitro intrinsic clearance rates for 54 chemicals tested using isolated hepatocytes from humans, rats, and rainbow trout. The test chemicals were selected to provide broad coverage across the industrial and pesticidal chemical space while also prioritizing chemicals of interest to EPA’s Endocrine Disruptor Screening Program (EDSP). A data evaluation framework was developed to identify results suitable for rate reporting. Acceptable results were then used to evaluate the chemical domain of applicability of the applied methods, the influence of starting substrate concentration on measured rates of intrinsic clearance, and differences in metabolic activity among species. These findings provide data for chemicals of specific interest to the EDSP. More importantly, the results provide critical guidance on future use of in vitro biotransformation assays to support high-throughput chemical risks assessments. This dataset is associated with the following publication: Black, S., J. Nichols, K. Fay, S. Matten, and S. Lynn. Evaluation and comparison of in vitro intrinsic hepatic clearance rates measured using cryopreserved hepatocytes from humans, rats, and rainbow trout. TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 457: 152819, (2021). |
| distribution |
[
{
"title": "Black et al_ScID A-4b8t_Dataset_20210129_entry.xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1520744/Black%20et%20al_ScID%20A-4b8t_Dataset_20210129_entry.xlsx"
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|
| identifier | https://doi.org/10.23719/1520744 |
| keyword |
[
"biotransformation",
"hepatocytes",
"in vitro in vivo extrapolation",
"in vitro intrinsic clearance"
]
|
| license | https://pasteur.epa.gov/license/sciencehub-license.html |
| modified | 2021-01-29 |
| programCode |
[
"020:095"
]
|
| publisher |
{
"name": "U.S. EPA Office of Research and Development (ORD)",
"subOrganizationOf": {
"name": "U.S. Environmental Protection Agency",
"subOrganizationOf": {
"name": "U.S. Government"
}
}
}
|
| references |
[
"https://doi.org/10.1016/j.tox.2021.152819"
]
|
| rights |
null
|
| title | Black et al_human rat and trout CLint_ScienceHub entry |
