• Organization: U.S. Department of Health & Human Services
• Updated: 2025-09-06

Background Biochemical and histochemical studies have both previously indicated plasma membrane-associated carbonic anhydrase (CA) activity in hepatocytes which has been assumed to be CA...

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• Organization: U.S. Department of Health & Human Services
• Updated: 2025-09-29

Previous studies have shown that several agents that stimulate heptahelical G-protein coupled receptors activate the extracellular signal regulated kinases ERK1 (p44mapk) and ERK2 (p42mapk) in...

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• Organization: U.S. Environmental Protection Agency
• Updated: 2020-09-09

This repository contains the necessary data, python source code and jupyter notebooks to reproduce the results from our manuscript, "Estimating Hepatotoxic Doses Using High-content Imaging in...

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• Organization: U.S. Environmental Protection Agency
• Updated: 2016-04-18

The purpose of this study was to compare two in vitro systems, cryopreserved trout hepatocytes and trout liver S9 fractions, used to predict in vivo levels of biotransformation in fish. This...

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• Organization: U.S. Department of Health & Human Services
• Updated: 2025-09-06

Background Activation of the extracellular signal-regulated kinases ERK1 and ERK2 in hepatocytes by prostaglandin (PG)F2α was recently found to be inhibited by pertussis toxin (PTX)...

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• Organization: U.S. Environmental Protection Agency
• Updated: 2020-04-15

RNA-seq data from HepaRG cells exposed for 2 hours to microcystin-LR and -RR at multiple concentrations each. This dataset is associated with the following publication: Biales, A., D. Bencic, R....

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• Organization: U.S. Environmental Protection Agency
• Updated: 2021-01-29

This dataset provides measured in vitro intrinsic clearance rates for 54 chemicals tested using isolated hepatocytes from humans, rats, and rainbow trout. The test chemicals were selected to...

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• Organization: U.S. Environmental Protection Agency
• Updated: 2020-06-09

We introduce a new high-throughput transcriptomics (HTTr) platform comprised of a collagen sandwich primary rat hepatocyte culture and the TempO-Seq assay for screening and prioritizing potential...

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• Organization: U.S. Environmental Protection Agency
• Updated: 2018-02-23

This paper describes the results of an international ring trial (round robin study) of in vitro methods used to measure chemical biotransformation in fish. Researchers at six laboratories in the...

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• Organization: U.S. Department of Health & Human Services
• Updated: 2025-09-06

Background Numerous investigations demonstrate a novel role of thyroid hormone as a modulator of signal transduction. Protein kinase C (PKC) is critical to the mechanism by which thyroid...

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• Organization: U.S. Department of Health & Human Services
• Updated: 2025-09-06

Background Thyroid hormones are well known modulators of signal transduction. The effect of hyper- and hypo-thyroidism on diacylglycerol/protein kinase C (DAG/PKC) signaling in...

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• Organization: U.S. Environmental Protection Agency
• Updated: 2019-11-19

Gene expression data from the Fluidigm qRT-PCR arrays was analyzed in R (v3.6.1; R Foundation for Statistical Computing, 2019). Prior to processing through the tcpl package, each qRT-PCR primer...

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• Organization: U.S. Environmental Protection Agency
• Updated: 2014-01-01

Background: Chemical toxicity testing is being transformed by advances in biology and computer modeling, concerns over animal use and the thousands of environmental chemicals lacking toxicity...