Comparison of cryopreserved trout hepatocytes and liver S9 fractions as in vitro tools for bioaccumulation assessment of chemicals that undergo biotransformation in fish
The purpose of this study was to compare two in vitro systems, cryopreserved trout hepatocytes and trout liver S9 fractions, used to predict in vivo levels of biotransformation in fish. This information is needed to refine modeled estimates of bioaccumulation for hydrophobic organic chemicals that undergo biotransformation. In this effort we used trout hepatocytes to measure in vitro biotransformation of 6 polycyclic aromatic hydrocarbons (PAHs). The results were compared to metabolism rates reported previously for trout liver S9 fractions. Results obtained using both in vitro systems were then used to predict measured levels of hepatic clearance for the same test chemicals exhibited by isolated perfused livers. The results of this study suggest that both in vitro systems are well suited for performing in vitro-in vivo metabolism extrapolations with fish as a means for improving modeled bioaccumulation predictions.
This dataset is associated with the following publication:
Fay, K., P. Fitzsimmons, A. Hoffman, and J. Nichols. Comparison of trout hepatocytes and liver S9 fractions as in vitro models for predicting hepatic clearance in fish. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, FL, USA, 36(2): 463-471, (2017).
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"020:00"
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| contactPoint |
{
"fn": "John Nichols",
"hasEmail": "mailto:nichols.john@epa.gov"
}
|
| description | The purpose of this study was to compare two in vitro systems, cryopreserved trout hepatocytes and trout liver S9 fractions, used to predict in vivo levels of biotransformation in fish. This information is needed to refine modeled estimates of bioaccumulation for hydrophobic organic chemicals that undergo biotransformation. In this effort we used trout hepatocytes to measure in vitro biotransformation of 6 polycyclic aromatic hydrocarbons (PAHs). The results were compared to metabolism rates reported previously for trout liver S9 fractions. Results obtained using both in vitro systems were then used to predict measured levels of hepatic clearance for the same test chemicals exhibited by isolated perfused livers. The results of this study suggest that both in vitro systems are well suited for performing in vitro-in vivo metabolism extrapolations with fish as a means for improving modeled bioaccumulation predictions. This dataset is associated with the following publication: Fay, K., P. Fitzsimmons, A. Hoffman, and J. Nichols. Comparison of trout hepatocytes and liver S9 fractions as in vitro models for predicting hepatic clearance in fish. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, FL, USA, 36(2): 463-471, (2017). |
| distribution |
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{
"title": "Fay et al 2016_IPL_Hep_Comparison_ Science Hub Data Summary_final.xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://pasteur.epa.gov/uploads/195/Fay%20et%20al%202016_IPL_Hep_Comparison_%20Science%20Hub%20Data%20Summary_final.xlsx"
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|
| identifier | A-b2rm-195 |
| keyword |
[
"bioaccumulation",
"biotransformation",
"fish",
"in vitro-in vivo extrapolation",
"rainbow trout",
"rainbow trout fish"
]
|
| license | https://pasteur.epa.gov/license/sciencehub-license.html |
| modified | 2016-04-18 |
| programCode |
[
"020:095"
]
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| publisher |
{
"name": "U.S. EPA Office of Research and Development (ORD)",
"subOrganizationOf": {
"name": "U.S. Environmental Protection Agency",
"subOrganizationOf": {
"name": "U.S. Government"
}
}
}
|
| references |
[
"https://doi.org/10.1002/etc.3572"
]
|
| rights |
null
|
| title | Comparison of cryopreserved trout hepatocytes and liver S9 fractions as in vitro tools for bioaccumulation assessment of chemicals that undergo biotransformation in fish |
