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Conserved B cell signaling, activation, and differentiation in porcine jejunal and ileal Peyer's patches despite distinct immune landscapes

Published by Agricultural Research Service | Department of Agriculture | Metadata Last Checked: January 27, 2026 | Last Modified: 2025-11-21
This dataset contains files used in the manuscript, "Conserved B cell signaling, activation, and differentiation in porcine jejunal and ileal Peyer's patches despite distinct immune landscapes" by Wiarda et al. (2023). Samples of jejunum and ileum containing Peyer's patches were collected from each of three, 8-week-old, female pigs and processed for single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (STomics). Data were analyzed to determine cell type compositions, spatial regions, gene expression, signaling interaction networks, activation trajectories, and cell locations. Files included here can be used to reconstruct all analyses of the manuscript, allowing individual users to perform independent queries. Also included is a vignette of all coding steps used in this work, provided as a Markdown file. Scripts and raw data will be available via preprint (to follow). See README for list of resources in this dataset with descriptions.

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Complete Metadata
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accessLevel public
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                                            [
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contactPoint 
                                            {
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}
description <p>This dataset contains files used in the manuscript, "Conserved B cell signaling, activation, and differentiation in porcine jejunal and ileal Peyer's patches despite distinct immune landscapes" by Wiarda et al. (2023). Samples of jejunum and ileum containing Peyer's patches were collected from each of three, 8-week-old, female pigs and processed for single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (STomics). Data were analyzed to determine cell type compositions, spatial regions, gene expression, signaling interaction networks, activation trajectories, and cell locations. Files included here can be used to reconstruct all analyses of the manuscript, allowing individual users to perform independent queries. Also included is a vignette of all coding steps used in this work, provided as a Markdown file. Scripts and raw data will be available via preprint (to follow).</p> <p>See README for list of resources in this dataset with descriptions.</p>
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identifier 10.15482/USDA.ADC/1529557
keyword 
                                            [
  "10X",
  "ARS",
  "B cell",
  "B cell subpopulations",
  "NP108",
  "Peyer's patch",
  "Pig",
  "Porcine",
  "Porcine leukocytes",
  "RNA sequencing",
  "RNA-Seq",
  "STomics",
  "Swine",
  "Visium",
  "chromium",
  "data.gov",
  "ileum",
  "immune",
  "intestine",
  "jejunum",
  "leukocyte",
  "leukocytes",
  "lymphocyte",
  "scRNA-seq",
  "single-cell",
  "single-cell RNA sequencing",
  "spatial",
  "spatial transcriptomics",
  "trancriptomics"
]
license https://creativecommons.org/licenses/by-sa/4.0/
modified 2025-11-21
programCode 
                                            [
  "005:040"
]
publisher 
                                            {
  "name": "Agricultural Research Service",
  "@type": "org:Organization"
}
spatial 
                                            "{"type": "Point", "coordinates": [-93.576693534851, 42.045130793063]}"
temporal 2020-04-01/2020-04-01
title Conserved B cell signaling, activation, and differentiation in porcine jejunal and ileal Peyer's patches despite distinct immune landscapes

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