Construction of a Dual-enzyme, Subzero (-30 degree C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry

                                            [
  "006:55"
]
                                        

                                            {
  "fn": "Jeffrey W. Hudgens",
  "hasEmail": "mailto:jeffrey.hudgens@nist.gov"
}
                                        

                                            [
  {
    "title": "DOI Access for Construction of a Dual-enzyme, Subzero (-30 degree C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry",
    "accessURL": "https://doi.org/10.18434/M32151"
  },
  {
    "title": "STL Files for parts used to construct the Multi-channel Temperature Controller",
    "format": "7Z",
    "mediaType": "application/octet-stream",
    "description": "STL Files for parts used to construct the Multi-channel Temperature Controller",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20T-controller%20STL%20Files.7z"
  },
  {
    "title": "ADDENDUM for HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 oC",
    "format": "word processing document",
    "mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document",
    "description": "This addendum provides information for configuring the instrument with an 8-port valve in the Valve #2 location. The 8-port valve offers the ability to backflush the protease, trap, and analytical columns, which allows more rapid removal of protease aggregates from the top of each column.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/ADDENDUM%20for%20HDX.docx"
  },
  {
    "title": "Multi-Channel Valve Controller STL files",
    "mediaType": "application/octet-stream",
    "description": "This file contains the STL files for the 3D-printed parts used to construct the Multi-Channel Valve Controller",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20Valve%20Controller%20STL%20files.7z"
  },
  {
    "title": "Multi-Channel Valve Controller",
    "mediaType": "application/pdf",
    "description": "This file presents mechanical drawings, electrical schematics, parts lists, STL file inventory for 3D-printed parts, and instructions for construction of the Multi-Channel Valve Controller.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20Valve%20Controller.pdf"
  },
  {
    "title": "Construction information for the Multi-channel Temperature Controller",
    "mediaType": "application/pdf",
    "description": "This file presents mechanical drawings, electrical schematics, parts lists, STL file inventory for 3D-printed parts, and instructions for construction of the Multi-channel Temperature Controller",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20T-controller.pdf"
  },
  {
    "title": "HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 degree C",
    "mediaType": "application/pdf",
    "description": "This file presents mechanical drawings, electrical schematics, parts lists, lists of STL files for 3D-printed parts, lists of IGS files for automated machining, and instructions for construction of the HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 degree C",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/HDX-MS%20Analytical%20Instrument.pdf"
  },
  {
    "title": "STL and IGS files that accompany the file, HDX-MS Analytical Instrument.pdf",
    "format": "7Z",
    "mediaType": "application/octet-stream",
    "description": "This is the compressed file containing the STL and IGS files that accompany the file, HDX-MS Analytical Instrument.pdf.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/HDX-MS%20Analytical%20Instrument%20STL-IGS%20Files.7z"
  },
  {
    "title": "SHA256 File for ADDENDUM for HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 oC",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/ADDENDUM%20for%20HDX.docx.sha256"
  },
  {
    "title": "SHA256 File for STL and IGS files that accompany the file, HDX-MS Analytical Instrument.pdf",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/HDX-MS%20Analytical%20Instrument%20STL-IGS%20Files.7z.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 degree C",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/HDX-MS%20Analytical%20Instrument.pdf.sha256"
  },
  {
    "title": "SHA256 File for STL Files for parts used to construct the Multi-channel Temperature Controller",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20T-controller%20STL%20Files.7z.sha256"
  },
  {
    "title": "SHA256 File for Construction information for the Multi-channel Temperature Controller",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20T-controller.pdf.sha256"
  },
  {
    "title": "SHA256 File for Multi-Channel Valve Controller STL files",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20Valve%20Controller%20STL%20files.7z.sha256"
  },
  {
    "title": "SHA256 File for Multi-Channel Valve Controller",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20Valve%20Controller.pdf.sha256"
  },
  {
    "mediaType": "application/pdf",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/T-controller%20%28ver%202%29.pdf"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/T-controller%20%28ver%202%29.pdf.sha256"
  }
]
                                        

                                            [
  "hydrogen-deuterium exchange",
  "liquid chromatography",
  "mass spectrometry",
  "peptide",
  "precision",
  "protein",
  "proteolysis",
  "proteomics",
  "reference material",
  "temperature control."
]
                                        

                                            [
  "en"
]
                                        

                                            [
  "006:045"
]
                                        

                                            {
  "name": "National Institute of Standards and Technology",
  "@type": "org:Organization"
}
                                        

                                            [
  "Bioscience:Bioprocessing",
  "Bioscience:Proteomics",
  "Chemistry:Analytical chemistry",
  "Metrology:Amount of substance",
  "Physics:Biological physics"
]