Effects of the antimicrobial contaminant triclocarban, and co-exposure with the androgen 17beta-trenbolone, on reproductive function and ovarian transcriptome of the fathead minnow (Pimephales promelas).

Triclocarban (TCC) is a widely used antimicrobial agent that is routinely detected in surface waters. The present study was designed to examine TCC’s efficacy and mode of action as a reproductive toxicant in fish. Reproductively mature Pimephales promelas were continuously exposed to either 1 or 5 μg TCC/L, 0.5 μg 17β-trenbolone (TRB)/L or a mixture (MIX) of 5 μg TCC and 0.5 μg TRB/L for 22 d and a variety of reproductive and endocrine-related endpoints were examined. The data set includes: -Concentrations of the test chemicals detected in water and tissues of exposed fish -Ex vivo production of testosterone and estradiol by gonad tissue placed in culture (ex vivo). -Plasma concentrations of testosterone, 17beta estradiol, and vitellogenin -Targeted gene expression measurements examining relative abundance of messenger RNA coding for enzymes involved in steroid synthesis: cholesterol side-chain cleavage (cyp11a), 17-a-hydroxylase/17,20 lyase (cyp17), aromatase (cyp19a1a), 3b-hydroxysteroid dehydrogenase (3bhsd), 11bhydroxysteroid dehydrogenase (11bhsd), and 17b-hydroxysteroid dehydrogenase (17bhsd) as well as five additional transcripts measured included steroidogenic acute regulatory protein (star), Vtg receptor (vtgr), follicle-stimulating hormone receptor (fshr), luteinizing hormone receptor (lhr), and androgen receptor (ar). -Ovarian transcriptomics data measured using a 15000 feature oligonucleotide microarray (GEO Platform Accession GPL10259). -Survival, reproduction, and morphological data. -. This dataset is associated with the following publication: Villeneuve , D., K. Jensen , J. Cavallin , E. Durhan, N. Garcia-Reyero, M. Kahl , R. Leino, E. Makynen, L. Wehmas, E. Perkins, and G. Ankley. Effects of the anti-microbial contaminant triclocarban and co-exposure with the androgen 17â-trenbolone, on reproductive function and ovarian transcriptome of the fathead minnow (Pimephales promelas). ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 36(1): :231-242, (2017).

                                            [
  "020:00"
]
                                        

                                            {
  "fn": "Daniel Villeneuve",
  "hasEmail": "mailto:villeneuve.dan@epa.gov"
}
                                        

                                            [
  {
    "title": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE64291",
    "accessURL": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE64291"
  },
  {
    "title": "ORD-011209 Triclocarban Science Hub Data File.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1503126/ORD-011209%20Triclocarban%20Science%20Hub%20Data%20File.xlsx"
  }
]
                                        

                                            [
  "adverse outcome pathway",
  "aquatic ecosystems",
  "ecotoxicology",
  "endocrine disruption",
  "screening and prioritization"
]
                                        

                                            [
  "020:095"
]
                                        

                                            {
  "name": "U.S. EPA Office of Research and Development (ORD)",
  "subOrganizationOf": {
    "name": "U.S. Environmental Protection Agency",
    "subOrganizationOf": {
      "name": "U.S. Government"
    }
  }
}
                                        

                                            [
  "https://doi.org/10.1002/etc.3531",
  "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6110301/"
]
                                        

                                            null