Measuring Microbial Community-Wide Antibiotic Resistance Propagation via Natural Transformation in the Human Gut Microbiome
This repository includes the raw data, analysis code, and results of a systematic analysis of natural transformation (i.e., horizontal gene transfer; HGT) of bacteria in a naturally occurring microbiome (human stool) following exposure to free extracellular DNA (eDNA) that coded for antibiotic resistance. A full description of this effort is available in the associated publication.
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | irregular |
| bureauCode |
[
"006:55"
]
|
| contactPoint |
{
"fn": "Samuel P. Forry",
"hasEmail": "mailto:samuel.forry@nist.gov"
}
|
| description | This repository includes the raw data, analysis code, and results of a systematic analysis of natural transformation (i.e., horizontal gene transfer; HGT) of bacteria in a naturally occurring microbiome (human stool) following exposure to free extracellular DNA (eDNA) that coded for antibiotic resistance. A full description of this effort is available in the associated publication. |
| distribution |
[
{
"title": "Figure 1 - ddPCR Analysis",
"mediaType": "application/octet-stream",
"description": "Analysis code of ddPCR data.",
"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Analysis&Visualization/ddPCR/FIG1-ddPCR-Analysis.Rmd"
},
{
"title": "README",
"mediaType": "text/plain",
"description": "This READEME file describes the organization this data record.",
"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/00README.txt.sha256"
},
{
"title": "Read Me",
"mediaType": "application/octet-stream",
"description": "This READEME file describes the organization this data record.",
"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Analysis&Visualization/CFU/FIG2-CFU-Analysis.Rmd"
},
{
"title": "Data",
"mediaType": "application/zip",
"description": "This compressed folder contains the raw data collected in the analysis of aforementioned samples. This includes CFU count results, ddPCR gene quantification, long-read next-generation sequencing (Nanopore) of isolates, and shotgun metagenomic sequencing characterization of microbiome samples.",
"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Data.zip"
},
{
"title": "READEME file",
"mediaType": "text/plain",
"description": "This file describes the organization of this data record.",
"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/00README.txt"
},
{
"title": "README",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Analysis&Visualization/CFU/FIG2-CFU-Analysis.Rmd.sha256"
},
{
"mediaType": "application/octet-stream",
"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Analysis&Visualization/WGS%20-%20Illumina/FIG4-WGS-AnalyzingProcessedReads.Rmd"
},
{
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Analysis&Visualization/WGS%20-%20Illumina/FIG4-WGS-AnalyzingProcessedReads.Rmd.sha256"
},
{
"mediaType": "application/octet-stream",
"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Analysis&Visualization/WGS%20-%20Illumina/FIG5-WGS-AnalyzingProcessedReads.Rmd"
},
{
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Analysis&Visualization/WGS%20-%20Illumina/FIG5-WGS-AnalyzingProcessedReads.Rmd.sha256"
},
{
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Analysis&Visualization/ddPCR/FIG1-ddPCR-Analysis.Rmd.sha256"
},
{
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/Data.zip.sha256"
},
{
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/HGT_STORMS_Checklist.xlsx"
},
{
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-3579/HGT_STORMS_Checklist.xlsx.sha256"
}
]
|
| identifier | ark:/88434/mds2-3579 |
| issued | 2024-11-07 |
| keyword |
[
"antibiotic resistance",
"horizontal gene transfer",
"microbiome",
"natural transformation"
]
|
| landingPage | https://data.nist.gov/od/id/mds2-3579 |
| language |
[
"en"
]
|
| license | https://www.nist.gov/open/license |
| modified | 2024-09-26 00:00:00 |
| programCode |
[
"006:045"
]
|
| publisher |
{
"name": "National Institute of Standards and Technology",
"@type": "org:Organization"
}
|
| theme |
[
"Bioscience:Microbial measurements"
]
|
| title | Measuring Microbial Community-Wide Antibiotic Resistance Propagation via Natural Transformation in the Human Gut Microbiome |
