Skip to main content
U.S. flag

An official website of the United States government

This site is currently in beta, and your feedback is helping shape its ongoing development.

Return to search results

RGTM 10169

Published by National Institute of Standards and Technology | National Institute of Standards and Technology | Metadata Last Checked: August 02, 2025 | Last Modified: 2022-07-15 00:00:00
NIST developed a reference material consisting of synthetic fragments of the SARS-CoV-2 virus RNA, which is the target of molecular diagnostic tests. These RNA fragments can assist in the development and validation of RT-qPCR assays for the detection SARS-CoV-2. The RNA fragments are characterized for concentration using digital PCR methods, may be used to assess limits of detection for SARS-CoV-2 assays, and may calibrate other in-house or commercial SARS-CoV-2 controls. This dataset includes data used for RTGM 10169 SARs-Cov-2 Research Grade Test Material validation. The Illumina and ONT sequence data were used to verify the construct sequence.

Resources

83 resources available

Find Related Datasets

Click any tag below to search for similar datasets

Complete Metadata
@type dcat:Dataset
accessLevel public
accrualPeriodicity irregular
bureauCode 
                                            [
  "006:55"
]
contactPoint 
                                            {
  "fn": "Megan Cleveland",
  "hasEmail": "mailto:megan.cleveland@nist.gov"
}
description NIST developed a reference material consisting of synthetic fragments of the SARS-CoV-2 virus RNA, which is the target of molecular diagnostic tests. These RNA fragments can assist in the development and validation of RT-qPCR assays for the detection SARS-CoV-2. The RNA fragments are characterized for concentration using digital PCR methods, may be used to assess limits of detection for SARS-CoV-2 assays, and may calibrate other in-house or commercial SARS-CoV-2 controls. This dataset includes data used for RTGM 10169 SARs-Cov-2 Research Grade Test Material validation. The Illumina and ONT sequence data were used to verify the construct sequence.
distribution 
                                            [
  {
    "title": "Research Grade Test Material (RGTM) 10169",
    "accessURL": "https://github.com/usnistgov/RGTM10169",
    "description": "information related to Research Grade Test Material (RGTM) 10169"
  },
  {
    "title": "ont_pycoqc.html",
    "mediaType": "application/x-zip-compressed",
    "description": "This file contains the ont_pycoqc.html file",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2714/ont%20pycoqc%20html.zip"
  },
  {
    "title": "multiqc.html",
    "mediaType": "application/x-zip-compressed",
    "description": "This zip file contains multiqc.html",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2714/multiqc%20html.zip"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/README.md"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/README.md.sha256"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/bam/ill_c1.txt"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/bam/ill_c1.txt.sha256"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/bam/ill_c2.txt"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/bam/ill_c2.txt.sha256"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/bam/ont.txt"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/bam/ont.txt.sha256"
  },
  {
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/fq/ill_c1_R1_fastqc.zip"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/fq/ill_c1_R1_fastqc.zip.sha256"
  },
  {
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/fq/ill_c1_R2_fastqc.zip"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/fq/ill_c1_R2_fastqc.zip.sha256"
  },
  {
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/fq/ill_c2_R1_fastqc.zip"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/fq/ill_c2_R1_fastqc.zip.sha256"
  },
  {
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/fq/ill_c2_R2_fastqc.zip"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/fq/ill_c2_R2_fastqc.zip.sha256"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc.log"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc.log.sha256"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_citations.txt"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_citations.txt.sha256"
  },
  {
    "mediaType": "application/json",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_data.json"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_data.json.sha256"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_fastqc.txt"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_fastqc.txt.sha256"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_general_stats.txt"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_general_stats.txt.sha256"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_samtools_stats.txt"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_samtools_stats.txt.sha256"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_sources.txt"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_sources.txt.sha256"
  },
  {
    "mediaType": "application/gzip",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/c1_R1_001.fastq.gz"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/c1_R1_001.fastq.gz.sha256"
  },
  {
    "mediaType": "application/gzip",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/c1_R2_001.fastq.gz"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/c1_R2_001.fastq.gz.sha256"
  },
  {
    "mediaType": "application/gzip",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/c2_R1_001.fastq.gz"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/c2_R1_001.fastq.gz.sha256"
  },
  {
    "mediaType": "application/gzip",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/c2_R2_001.fastq.gz"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/c2_R2_001.fastq.gz.sha256"
  },
  {
    "mediaType": "application/octet-stream",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill.bam"
  },
  {
    "mediaType": "application/octet-stream",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill.bam.bai"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill.bam.bai.sha256"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill.bam.sha256"
  },
  {
    "mediaType": "application/gzip",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_c1.vcf.gz"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_c1.vcf.gz.sha256"
  },
  {
    "mediaType": "application/octet-stream",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_c1.vcf.gz.tbi"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_c1.vcf.gz.tbi.sha256"
  },
  {
    "mediaType": "application/gzip",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_c2.vcf.gz"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_c2.vcf.gz.sha256"
  },
  {
    "mediaType": "application/octet-stream",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_c2.vcf.gz.tbi"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_c2.vcf.gz.tbi.sha256"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_depth.txt"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_depth.txt.sha256"
  },
  {
    "mediaType": "application/octet-stream",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/md5.chk"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/md5.chk.sha256"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/md5sum_list.txt"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/md5sum_list.txt.sha256"
  },
  {
    "mediaType": "application/gzip",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/fast5s.tar.gz"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/fast5s.tar.gz.sha256"
  },
  {
    "mediaType": "application/octet-stream",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont.bam"
  },
  {
    "mediaType": "application/octet-stream",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont.bam.bai"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont.bam.bai.sha256"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont.bam.sha256"
  },
  {
    "mediaType": "application/gzip",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont.fastq.gz"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont.fastq.gz.sha256"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont_depth.txt"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont_depth.txt.sha256"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont_guppy_sequencing_summary.txt"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont_guppy_sequencing_summary.txt.sha256"
  },
  {
    "mediaType": "application/octet-stream",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/annotations.bed"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/annotations.bed.sha256"
  },
  {
    "mediaType": "application/octet-stream",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/construct1_w_flankings.fa"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/construct1_w_flankings.fa.sha256"
  },
  {
    "mediaType": "application/octet-stream",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/construct2_w_flankings.fa"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/construct2_w_flankings.fa.sha256"
  },
  {
    "mediaType": "application/octet-stream",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/constructs_w_flankings.fa"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/constructs_w_flankings.fa.sha256"
  },
  {
    "mediaType": "application/octet-stream",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/primers.bed"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/primers.bed.sha256"
  },
  {
    "mediaType": "application/octet-stream",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/sars-cov-2-RGTM.genome"
  },
  {
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/sars-cov-2-RGTM.genome.sha256"
  }
]
identifier ark:/88434/mds2-2714
issued 2023-02-09
keyword 
                                            [
  "Biosciences and Health",
  "DNA",
  "PCR",
  "SRM",
  "digital PCR",
  "reference material",
  "sequencing",
  "virus"
]
landingPage https://data.nist.gov/od/id/mds2-2714
language 
                                            [
  "en"
]
license https://www.nist.gov/open/license
modified 2022-07-15 00:00:00
programCode 
                                            [
  "006:045"
]
publisher 
                                            {
  "name": "National Institute of Standards and Technology",
  "@type": "org:Organization"
}
theme 
                                            [
  "Bioscience:Genomic measurements"
]
title RGTM 10169

data.gov

An official website of the GSA's Technology Transformation Services

Looking for U.S. government information and services?
Visit USA.gov