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RGTM 10169

Published by National Institute of Standards and Technology | National Institute of Standards and Technology | Metadata Last Checked: August 02, 2025 | Last Modified: 2022-07-15 00:00:00
NIST developed a reference material consisting of synthetic fragments of the SARS-CoV-2 virus RNA, which is the target of molecular diagnostic tests. These RNA fragments can assist in the development and validation of RT-qPCR assays for the detection SARS-CoV-2. The RNA fragments are characterized for concentration using digital PCR methods, may be used to assess limits of detection for SARS-CoV-2 assays, and may calibrate other in-house or commercial SARS-CoV-2 controls. This dataset includes data used for RTGM 10169 SARs-Cov-2 Research Grade Test Material validation. The Illumina and ONT sequence data were used to verify the construct sequence.

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Complete Metadata
@type dcat:Dataset
accessLevel public
accrualPeriodicity irregular
bureauCode 
                                            [
  "006:55"
]
contactPoint 
                                            {
  "fn": "Megan Cleveland",
  "hasEmail": "mailto:megan.cleveland@nist.gov"
}
description NIST developed a reference material consisting of synthetic fragments of the SARS-CoV-2 virus RNA, which is the target of molecular diagnostic tests. These RNA fragments can assist in the development and validation of RT-qPCR assays for the detection SARS-CoV-2. The RNA fragments are characterized for concentration using digital PCR methods, may be used to assess limits of detection for SARS-CoV-2 assays, and may calibrate other in-house or commercial SARS-CoV-2 controls. This dataset includes data used for RTGM 10169 SARs-Cov-2 Research Grade Test Material validation. The Illumina and ONT sequence data were used to verify the construct sequence.
distribution 
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identifier ark:/88434/mds2-2714
issued 2023-02-09
keyword 
                                            [
  "Biosciences and Health",
  "DNA",
  "PCR",
  "SRM",
  "digital PCR",
  "reference material",
  "sequencing",
  "virus"
]
landingPage https://data.nist.gov/od/id/mds2-2714
language 
                                            [
  "en"
]
license https://www.nist.gov/open/license
modified 2022-07-15 00:00:00
programCode 
                                            [
  "006:045"
]
publisher 
                                            {
  "name": "National Institute of Standards and Technology",
  "@type": "org:Organization"
}
theme 
                                            [
  "Bioscience:Genomic measurements"
]
title RGTM 10169

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