Cross-species comparison of chemical inhibition of human and Xenopus iodotyrosine deiodinase
Supplementary materials for "Olker JH, Korte JJ, Haselman JT, Hornung MW, Degitz SJ. Cross-species comparison of chemical inhibition of human and Xenopus iodotyrosine deiodinase. Aquat Toxicol. 2022 Aug;249:106227. doi: 10.1016/j.aquatox.2022.106227. Epub 2022 Jun 15. PMID: 35767922; PMCID: PMC9887787."
The excel spreadsheet contains the resultant data from an assay for chemical inhibition of amphibian Iodotyrosine Deiodinase (IYD) enzyme activity. 154 chemicals from the EPA’s ToxCast chemical library were tested in concentration-response and these amphibian IYD assay results were compared to those from the human IYD inhibition assay reported in Olker et al. 2021 (doi:10.1016/j.tiv.2020.105073). The same set of 154 chemicals were tested in both assays and compared here. This data set the median, minimum, and maximum inhibition produced at each concentration for each tested chemical. A model inhibitor (3-Nitro-L-Tyrosine) was included on each plate as a positive control, with concentration response data for those curves also included in the data set.
This dataset is associated with the following publication:
Olker, J., J. Korte, J. Haselman, M. Hornung, and S. Degitz. Xenopus laevis and Human iodotyrosine deiodinase enzyme cross-species sensitivity to inhibition by ToxCast chemicals.. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 249: N/A, (2022).
Resources
2 resources available
-
Appendix A - Supplementary Data.pdf
APPLICATION/PDF -
Olker_et_al_IYD_in vitro Species Comp_210709.xlsx
APPLICATION/VND.OPENXMLFORMATS-OFFICEDOCUMENT.SPREADSHEETML.SHEET
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"020:00"
]
|
| contactPoint |
{
"fn": "Jennifer Olker",
"hasEmail": "mailto:olker.jennifer@epa.gov"
}
|
| description | Supplementary materials for "Olker JH, Korte JJ, Haselman JT, Hornung MW, Degitz SJ. Cross-species comparison of chemical inhibition of human and Xenopus iodotyrosine deiodinase. Aquat Toxicol. 2022 Aug;249:106227. doi: 10.1016/j.aquatox.2022.106227. Epub 2022 Jun 15. PMID: 35767922; PMCID: PMC9887787." The excel spreadsheet contains the resultant data from an assay for chemical inhibition of amphibian Iodotyrosine Deiodinase (IYD) enzyme activity. 154 chemicals from the EPA’s ToxCast chemical library were tested in concentration-response and these amphibian IYD assay results were compared to those from the human IYD inhibition assay reported in Olker et al. 2021 (doi:10.1016/j.tiv.2020.105073). The same set of 154 chemicals were tested in both assays and compared here. This data set the median, minimum, and maximum inhibition produced at each concentration for each tested chemical. A model inhibitor (3-Nitro-L-Tyrosine) was included on each plate as a positive control, with concentration response data for those curves also included in the data set. This dataset is associated with the following publication: Olker, J., J. Korte, J. Haselman, M. Hornung, and S. Degitz. Xenopus laevis and Human iodotyrosine deiodinase enzyme cross-species sensitivity to inhibition by ToxCast chemicals.. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 249: N/A, (2022). |
| distribution |
[
{
"title": "Appendix A - Supplementary Data.pdf",
"mediaType": "application/pdf",
"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1530078/Appendix%20A%20-%20Supplementary%20Data.pdf"
},
{
"title": "Olker_et_al_IYD_in vitro Species Comp_210709.xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1530078/Olker_et_al_IYD_in%20vitro%20Species%20Comp_210709.xlsx"
}
]
|
| identifier | https://doi.org/10.23719/1530078 |
| keyword |
[
"Xenopus",
"amphibian",
"endocrine disruption",
"iodotyrosine deiodinase",
"screening",
"thyroid"
]
|
| license | https://pasteur.epa.gov/license/sciencehub-license.html |
| modified | 2022-06-14 |
| programCode |
[
"020:000"
]
|
| publisher |
{
"name": "U.S. EPA Office of Research and Development (ORD)",
"subOrganizationOf": {
"name": "U.S. Environmental Protection Agency",
"subOrganizationOf": {
"name": "U.S. Government"
}
}
}
|
| references |
[
"https://doi.org/10.1016/j.aquatox.2022.106227",
"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9887787"
]
|
| rights |
null
|
| title | Cross-species comparison of chemical inhibition of human and Xenopus iodotyrosine deiodinase |
