Data from: Gene expression during the early stages of host perception and attachment in adult female Rhipicephalus microplus ticks
This study produced a comprehensive transcriptome from newly molted adult ticks and will provide a useful resource for studies of tick feeding and host perception and also assist genome annotation refinements.
The goal was to develop a comprehensive transcriptome of adult female Rhipicephalus microplus ticks and discover transcriptome changes that are related to feeding and host-perception prior to feeding. We have sequenced the transcriptomes of unfed newly molted adult female ticks, newly molted adult females that were feeding upon a bovine host for 3 hours following attachment to the host, and newly molted adult females that were placed in a gas-permeable bag attached to a bovine host for 3 hours (attachment to host prevented but host odors available to the ticks). There were 2 biological replicates and transcriptomes assembled from each individual experimental condition. A collective adult female transcriptome was assembled from sequence data pooled from all 3 conditions (using data from all replicates). Differential gene expression was examined using all possible comparisons to detect putative feeding-associated and host perception-associated transcripts. Resources in this dataset:Resource Title: Gene expression during the early stages of host perception and attachment in adult female Rhipicephalus microplus ticks. File Name: Web Page, url: https://doi.org/10.1007/s10493-019-00420-1
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[
"005:18"
]
|
| contactPoint |
{
"fn": "Bendele, Kylie",
"hasEmail": "mailto:Kylie.Bendele@usda.gov"
}
|
| description | <p>This study produced a comprehensive transcriptome from newly molted adult ticks and will provide a useful resource for studies of tick feeding and host perception and also assist genome annotation refinements.</p> <p>The goal was to develop a comprehensive transcriptome of adult female Rhipicephalus microplus ticks and discover transcriptome changes that are related to feeding and host-perception prior to feeding. We have sequenced the transcriptomes of unfed newly molted adult female ticks, newly molted adult females that were feeding upon a bovine host for 3 hours following attachment to the host, and newly molted adult females that were placed in a gas-permeable bag attached to a bovine host for 3 hours (attachment to host prevented but host odors available to the ticks). There were 2 biological replicates and transcriptomes assembled from each individual experimental condition. A collective adult female transcriptome was assembled from sequence data pooled from all 3 conditions (using data from all replicates). Differential gene expression was examined using all possible comparisons to detect putative feeding-associated and host perception-associated transcripts. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Gene expression during the early stages of host perception and attachment in adult female Rhipicephalus microplus ticks.</p> <p>File Name: Web Page, url: <a href="https://doi.org/10.1007/s10493-019-00420-1">https://doi.org/10.1007/s10493-019-00420-1</a> </p></li></ul><p></p> |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "https://doi.org/10.1007/s10493-019-00420-1",
"mediaType": "text/html",
"downloadURL": "https://doi.org/10.1007/s10493-019-00420-1"
}
]
|
| identifier | 10.1007/s10493-019-00420-1 |
| keyword |
[
"ARS",
"NP104",
"cattle tick",
"data.gov",
"feeding response",
"gene expression",
"host perception",
"transcriptomics"
]
|
| license | https://creativecommons.org/publicdomain/zero/1.0/ |
| modified | 2025-11-21 |
| programCode |
[
"005:040"
]
|
| publisher |
{
"name": "Agricultural Research Service",
"@type": "org:Organization"
}
|
| spatial |
"{"type": "Point", "coordinates": [-98.32857, 26.38684]}"
|
| temporal | 2008-11-17/2008-11-18 |
| title | Data from: Gene expression during the early stages of host perception and attachment in adult female Rhipicephalus microplus ticks |