Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor
This database gives hydrogen-deuterium exchange mass spectrometry (HDX-MS) data from measurements of three purified IgG1 glycoform samples, predominantly G0F, G2F, and SAF, in isolation and in complexation with the high-affinity receptor, Fc(gamma)RIa (CD64). The IgG1 antibody used in this study, aIL8hFc, is a murine-human chimeric IgG1, which inhibits IL-8 binding to human neutrophils. The working datasets comprise 17,750 deuterium uptake measurements in peptides from the receptor and the light and heavy chains of aIL8hFc obtained during 7 experiments, each comprising three runs of six exchange times. The data illuminate on the hydrogen exchange dynamics in isolated receptor, isolated IgG1 glycoforms, and receptor-IgG1 complexes.
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[
"006:55"
]
|
| contactPoint |
{
"fn": "Jeffrey W. Hudgens",
"hasEmail": "mailto:jeffrey.hudgens@nist.gov"
}
|
| description | This database gives hydrogen-deuterium exchange mass spectrometry (HDX-MS) data from measurements of three purified IgG1 glycoform samples, predominantly G0F, G2F, and SAF, in isolation and in complexation with the high-affinity receptor, Fc(gamma)RIa (CD64). The IgG1 antibody used in this study, aIL8hFc, is a murine-human chimeric IgG1, which inhibits IL-8 binding to human neutrophils. The working datasets comprise 17,750 deuterium uptake measurements in peptides from the receptor and the light and heavy chains of aIL8hFc obtained during 7 experiments, each comprising three runs of six exchange times. The data illuminate on the hydrogen exchange dynamics in isolated receptor, isolated IgG1 glycoforms, and receptor-IgG1 complexes. |
| distribution |
[
{
"title": "DOI Access for Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor",
"accessURL": "https://doi.org/10.18434/mds2-2365"
},
{
"title": "Glossary of Protein Modifications",
"mediaType": "application/pdf",
"description": "Glossary of post translational modifications present on peptides.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Glossary%20of%20Protein%20Modifications.pdf"
},
{
"title": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform.",
"format": "CSV",
"mediaType": "text/csv",
"description": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2314_holo-FcgR1a%28G0F%29.CSV"
},
{
"title": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform.",
"format": "CSV",
"mediaType": "text/csv",
"description": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2315_holo-FcgR1a%28G2F%29.CSV"
},
{
"title": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform.",
"format": "CSV",
"mediaType": "text/csv",
"description": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2316_holo-FcgR1a%28SAF%29.CSV"
},
{
"title": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a.",
"format": "CSV",
"mediaType": "text/csv",
"description": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%238_holo-G0F_heavy_chain%28FcgR1a%29.CSV"
},
{
"title": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
"format": "CSV",
"mediaType": "text/csv",
"description": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2310_holo-G2F_heavy_chain%28FcgR1a%29.CSV"
},
{
"title": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
"format": "CSV",
"mediaType": "text/csv",
"description": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2312_holo-SAF_heavy_chain%28FcgR1a%29.CSV"
},
{
"title": "HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a .",
"format": "CSV",
"mediaType": "text/csv",
"description": "HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%232_holo-G0F_light_chain%28FcgR1a%29.CSV"
},
{
"title": "HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
"format": "CSV",
"mediaType": "text/csv",
"description": "HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%234_holo-G2F_light_chain%28FcgR1a%29.CSV"
},
{
"title": "HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
"format": "CSV",
"mediaType": "text/csv",
"description": "HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%236_holo-SAF_light_chain%28FcgR1a%29.CSV"
},
{
"title": "HDX-MS data of peptides from isolated (apo-)Fc?R1a.",
"format": "CSV",
"mediaType": "application/vnd.ms-excel",
"description": "HDX-MS data of peptides from isolated (apo-)Fc?R1a.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2313_apo-FcgR1a.CSV"
},
{
"title": "HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc.",
"format": "CSV",
"mediaType": "text/csv",
"description": "HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%237_apo-G0F_heavy_chain.CSV"
},
{
"title": "HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc.",
"format": "CSV",
"mediaType": "text/csv",
"description": "HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%239_apo-G2F_heavy_chain.CSV"
},
{
"title": "HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc.",
"format": "CSV",
"mediaType": "text/csv",
"description": "HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2311_apo-SAF_heavy_chain.CSV"
},
{
"title": "HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc",
"format": "CSV",
"mediaType": "text/csv",
"description": "HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%231_apo-G0F_light_chain.CSV"
},
{
"title": "HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc",
"format": "csv",
"mediaType": "text/csv",
"description": "HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%233_apo-G2F_light_chain.CSV"
},
{
"title": "SHA256 File HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc",
"mediaType": "text/plain",
"description": "HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%233_apo-G2F_light_chain.CSV.sha256"
},
{
"title": "HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc",
"format": "CSV",
"mediaType": "text/csv",
"description": "HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%235_apo-SAF_light_chain.CSV"
},
{
"title": "SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
"mediaType": "text/plain",
"description": "SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%236_holo-SAF_light_chain%28FcgR1a%29.CSV.sha256"
},
{
"title": "Sequences of aILhFc and soluble FcgR1a (CD64a)",
"mediaType": "application/pdf",
"description": "Sequences of aILhFc and soluble FcgR1a (CD64a)",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Sequences%20of%20aILhFc%20and%20soluble%20FcgR1a%20%28CD64a%29.pdf"
},
{
"title": "SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2310_holo-G2F_heavy_chain%28FcgR1a%29.CSV.sha256"
},
{
"title": "SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc.",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2311_apo-SAF_heavy_chain.CSV.sha256"
},
{
"title": "SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2312_holo-SAF_heavy_chain%28FcgR1a%29.CSV.sha256"
},
{
"title": "SHA256 File for HDX-MS data of peptides from isolated (apo-)Fc(gamma)R1a.",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2313_apo-FcgR1a.CSV.sha256"
},
{
"title": "SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform.",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2314_holo-FcgR1a%28G0F%29.CSV.sha256"
},
{
"title": "SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform.",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2315_holo-FcgR1a%28G2F%29.CSV.sha256"
},
{
"title": "SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform.",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2316_holo-FcgR1a%28SAF%29.CSV.sha256"
},
{
"title": "SHA256 File for HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%231_apo-G0F_light_chain.CSV.sha256"
},
{
"title": "SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a .",
"mediaType": "text/csv",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%232_holo-G0F_light_chain%28FcgR1a%29.CSV.sha256"
},
{
"title": "SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%234_holo-G2F_light_chain%28FcgR1a%29.CSV.sha256"
},
{
"title": "SHA256 File for HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc",
"format": "CSV",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%235_apo-SAF_light_chain.CSV.sha256"
},
{
"title": "SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc.",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%237_apo-G0F_heavy_chain.CSV.sha256"
},
{
"title": "SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a.",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%238_holo-G0F_heavy_chain%28FcgR1a%29.CSV.sha256"
},
{
"title": "SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc.",
"format": "CSV",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%239_apo-G2F_heavy_chain.CSV.sha256"
},
{
"title": "SHA256 File for Glossary of Protein Modifications",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Glossary%20of%20Protein%20Modifications.pdf.sha256"
},
{
"title": "SHA256 File for Sequences of aILhFc and soluble FcgR1a (CD64a)",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Sequences%20of%20aILhFc%20and%20soluble%20FcgR1a%20%28CD64a%29.pdf.sha256"
}
]
|
| identifier | ark:/88434/mds2-2365 |
| issued | 2021-06-03 |
| keyword |
[
"HDX-MS",
"antibody-receptor interaction",
"chromatography",
"glycosylation",
"hydrogen-deuterium exchange",
"mass spectrometry",
"monoclonal antibody",
"peptide",
"precision",
"protein",
"proteolysis",
"proteomics",
"receptor."
]
|
| language |
[
"en"
]
|
| license | https://www.nist.gov/open/license |
| modified | 2021-02-17 00:00:00 |
| programCode |
[
"006:045"
]
|
| publisher |
{
"name": "National Institute of Standards and Technology",
"@type": "org:Organization"
}
|
| theme |
[
"Bioscience:Biomaterials",
"Bioscience:Proteomics",
"Chemistry:Analytical chemistry",
"Chemistry:Molecular characterization",
"Health:Pharmaceuticals",
"Manufacturing:Biomanufacturing",
"Materials:Materials characterization"
]
|
| title | Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor |
