Skip to main content
U.S. flag

An official website of the United States government

This site is currently in beta, and your feedback is helping shape its ongoing development.

Return to search results

Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor

Published by National Institute of Standards and Technology | National Institute of Standards and Technology | Metadata Last Checked: August 02, 2025 | Last Modified: 2021-02-17 00:00:00
This database gives hydrogen-deuterium exchange mass spectrometry (HDX-MS) data from measurements of three purified IgG1 glycoform samples, predominantly G0F, G2F, and SAF, in isolation and in complexation with the high-affinity receptor, Fc(gamma)RIa (CD64). The IgG1 antibody used in this study, aIL8hFc, is a murine-human chimeric IgG1, which inhibits IL-8 binding to human neutrophils. The working datasets comprise 17,750 deuterium uptake measurements in peptides from the receptor and the light and heavy chains of aIL8hFc obtained during 7 experiments, each comprising three runs of six exchange times. The data illuminate on the hydrogen exchange dynamics in isolated receptor, isolated IgG1 glycoforms, and receptor-IgG1 complexes.

Resources

37 resources available

  • DOI Access for Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor

    FILE
    Visit Page

  • Glossary of Protein Modifications

    APPLICATION/PDF
    Download

  • HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform.

    CSV
    Download

  • HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform.

    CSV
    Download

  • HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform.

    CSV
    Download

  • HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a.

    CSV
    Download

  • HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.

    CSV
    Download

  • HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.

    CSV
    Download

  • HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a .

    CSV
    Download

  • HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.

    CSV
    Download

  • HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.

    CSV
    Download

  • HDX-MS data of peptides from isolated (apo-)Fc?R1a.

    CSV
    Download

  • HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc.

    CSV
    Download

  • HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc.

    CSV
    Download

  • HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc.

    CSV
    Download

  • HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc

    CSV
    Download

  • HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc

    CSV
    Download

  • SHA256 File HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc

    TEXT/PLAIN
    Download

  • HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc

    CSV
    Download

  • SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.

    TEXT/PLAIN
    Download

  • Sequences of aILhFc and soluble FcgR1a (CD64a)

    APPLICATION/PDF
    Download

  • SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data of peptides from isolated (apo-)Fc(gamma)R1a.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a .

    TEXT/CSV
    Download

  • SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc

    CSV
    Download

  • SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a.

    TEXT/PLAIN
    Download

  • SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc.

    CSV
    Download

  • SHA256 File for Glossary of Protein Modifications

    TEXT/PLAIN
    Download

  • SHA256 File for Sequences of aILhFc and soluble FcgR1a (CD64a)

    TEXT/PLAIN
    Download

Find Related Datasets

Click any tag below to search for similar datasets

Complete Metadata
@type dcat:Dataset
accessLevel public
bureauCode 
                                            [
  "006:55"
]
contactPoint 
                                            {
  "fn": "Jeffrey W. Hudgens",
  "hasEmail": "mailto:jeffrey.hudgens@nist.gov"
}
description This database gives hydrogen-deuterium exchange mass spectrometry (HDX-MS) data from measurements of three purified IgG1 glycoform samples, predominantly G0F, G2F, and SAF, in isolation and in complexation with the high-affinity receptor, Fc(gamma)RIa (CD64). The IgG1 antibody used in this study, aIL8hFc, is a murine-human chimeric IgG1, which inhibits IL-8 binding to human neutrophils. The working datasets comprise 17,750 deuterium uptake measurements in peptides from the receptor and the light and heavy chains of aIL8hFc obtained during 7 experiments, each comprising three runs of six exchange times. The data illuminate on the hydrogen exchange dynamics in isolated receptor, isolated IgG1 glycoforms, and receptor-IgG1 complexes.
distribution 
                                            [
  {
    "title": "DOI Access for Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor",
    "accessURL": "https://doi.org/10.18434/mds2-2365"
  },
  {
    "title": "Glossary of Protein Modifications",
    "mediaType": "application/pdf",
    "description": "Glossary of post translational modifications present on peptides.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Glossary%20of%20Protein%20Modifications.pdf"
  },
  {
    "title": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2314_holo-FcgR1a%28G0F%29.CSV"
  },
  {
    "title": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2315_holo-FcgR1a%28G2F%29.CSV"
  },
  {
    "title": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2316_holo-FcgR1a%28SAF%29.CSV"
  },
  {
    "title": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%238_holo-G0F_heavy_chain%28FcgR1a%29.CSV"
  },
  {
    "title": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2310_holo-G2F_heavy_chain%28FcgR1a%29.CSV"
  },
  {
    "title": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2312_holo-SAF_heavy_chain%28FcgR1a%29.CSV"
  },
  {
    "title": "HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a .",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%232_holo-G0F_light_chain%28FcgR1a%29.CSV"
  },
  {
    "title": "HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%234_holo-G2F_light_chain%28FcgR1a%29.CSV"
  },
  {
    "title": "HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%236_holo-SAF_light_chain%28FcgR1a%29.CSV"
  },
  {
    "title": "HDX-MS data of peptides from isolated (apo-)Fc?R1a.",
    "format": "CSV",
    "mediaType": "application/vnd.ms-excel",
    "description": "HDX-MS data of peptides from isolated (apo-)Fc?R1a.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2313_apo-FcgR1a.CSV"
  },
  {
    "title": "HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%237_apo-G0F_heavy_chain.CSV"
  },
  {
    "title": "HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%239_apo-G2F_heavy_chain.CSV"
  },
  {
    "title": "HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc.",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2311_apo-SAF_heavy_chain.CSV"
  },
  {
    "title": "HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%231_apo-G0F_light_chain.CSV"
  },
  {
    "title": "HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc",
    "format": "csv",
    "mediaType": "text/csv",
    "description": "HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%233_apo-G2F_light_chain.CSV"
  },
  {
    "title": "SHA256 File HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc",
    "mediaType": "text/plain",
    "description": "HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%233_apo-G2F_light_chain.CSV.sha256"
  },
  {
    "title": "HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc",
    "format": "CSV",
    "mediaType": "text/csv",
    "description": "HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%235_apo-SAF_light_chain.CSV"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
    "mediaType": "text/plain",
    "description": "SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%236_holo-SAF_light_chain%28FcgR1a%29.CSV.sha256"
  },
  {
    "title": "Sequences of aILhFc and soluble FcgR1a (CD64a)",
    "mediaType": "application/pdf",
    "description": "Sequences of aILhFc and soluble FcgR1a (CD64a)",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Sequences%20of%20aILhFc%20and%20soluble%20FcgR1a%20%28CD64a%29.pdf"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2310_holo-G2F_heavy_chain%28FcgR1a%29.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2311_apo-SAF_heavy_chain.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2312_holo-SAF_heavy_chain%28FcgR1a%29.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data of peptides from isolated (apo-)Fc(gamma)R1a.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2313_apo-FcgR1a.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2314_holo-FcgR1a%28G0F%29.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2315_holo-FcgR1a%28G2F%29.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%2316_holo-FcgR1a%28SAF%29.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%231_apo-G0F_light_chain.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a .",
    "mediaType": "text/csv",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%232_holo-G0F_light_chain%28FcgR1a%29.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%234_holo-G2F_light_chain%28FcgR1a%29.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc",
    "format": "CSV",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%235_apo-SAF_light_chain.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%237_apo-G0F_heavy_chain.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a.",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%238_holo-G0F_heavy_chain%28FcgR1a%29.CSV.sha256"
  },
  {
    "title": "SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc.",
    "format": "CSV",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Dataset%239_apo-G2F_heavy_chain.CSV.sha256"
  },
  {
    "title": "SHA256 File for Glossary of Protein Modifications",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Glossary%20of%20Protein%20Modifications.pdf.sha256"
  },
  {
    "title": "SHA256 File for Sequences of aILhFc and soluble FcgR1a (CD64a)",
    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2365/Sequences%20of%20aILhFc%20and%20soluble%20FcgR1a%20%28CD64a%29.pdf.sha256"
  }
]
identifier ark:/88434/mds2-2365
issued 2021-06-03
keyword 
                                            [
  "HDX-MS",
  "antibody-receptor interaction",
  "chromatography",
  "glycosylation",
  "hydrogen-deuterium exchange",
  "mass spectrometry",
  "monoclonal antibody",
  "peptide",
  "precision",
  "protein",
  "proteolysis",
  "proteomics",
  "receptor."
]
language 
                                            [
  "en"
]
license https://www.nist.gov/open/license
modified 2021-02-17 00:00:00
programCode 
                                            [
  "006:045"
]
publisher 
                                            {
  "name": "National Institute of Standards and Technology",
  "@type": "org:Organization"
}
theme 
                                            [
  "Bioscience:Biomaterials",
  "Bioscience:Proteomics",
  "Chemistry:Analytical chemistry",
  "Chemistry:Molecular characterization",
  "Health:Pharmaceuticals",
  "Manufacturing:Biomanufacturing",
  "Materials:Materials characterization"
]
title Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor

data.gov

An official website of the GSA's Technology Transformation Services

Looking for U.S. government information and services?
Visit USA.gov