Development of an In Vitro Human Thyroid Microtissue Model for Chemical Screening
The objective of this study was to develop a medium-throughput organotypic screening assay comprised of reconstructed human thyroid microtissues to quantitatively evaluate the disruptive effects of chemicals on TH production and secretion. Primary human thyroid cells procured from qualified euthyroid donors were analyzed for retention of NK2 homeobox 1 (NKX2-1), Keratin 7 (KRT7), and Thyroglobulin (TG) expression by high-content image analysis to verify enrichment of follicular epithelial cells. A direct comparison of two-dimensional (2D) and three-dimensional (3D) 96-well culture formats
was employed to characterize the morphology, differential gene expression, TG production, and TH synthesis over the course of 20 days.
This dataset is associated with the following publication:
Deisenroth, C., V. Soldatow, J. Ford, W. Stewart, C. Brinkman, E. LeCluyse, D. Macmillan, and R. Thomas. Development of an In Vitro Human Thyroid Microtissue Model for Chemical Screening (ToxSci). TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 174(1): 63-78, (2020).
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"020:00"
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|
| contactPoint |
{
"fn": "Thomas Knudsen",
"hasEmail": "mailto:knudsen.thomas@epa.gov"
}
|
| description | The objective of this study was to develop a medium-throughput organotypic screening assay comprised of reconstructed human thyroid microtissues to quantitatively evaluate the disruptive effects of chemicals on TH production and secretion. Primary human thyroid cells procured from qualified euthyroid donors were analyzed for retention of NK2 homeobox 1 (NKX2-1), Keratin 7 (KRT7), and Thyroglobulin (TG) expression by high-content image analysis to verify enrichment of follicular epithelial cells. A direct comparison of two-dimensional (2D) and three-dimensional (3D) 96-well culture formats was employed to characterize the morphology, differential gene expression, TG production, and TH synthesis over the course of 20 days. This dataset is associated with the following publication: Deisenroth, C., V. Soldatow, J. Ford, W. Stewart, C. Brinkman, E. LeCluyse, D. Macmillan, and R. Thomas. Development of an In Vitro Human Thyroid Microtissue Model for Chemical Screening (ToxSci). TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 174(1): 63-78, (2020). |
| distribution |
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"title": "Figures_All_Deisenroth_TOCM Method_Final.docx",
"mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document",
"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1504187/Figures_All_Deisenroth_TOCM%20Method_Final.docx"
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|
| identifier | https://doi.org/10.23719/1504187 |
| keyword |
[
"endocrine toxicology",
"organotypic culture model",
"thyroid",
"thyroid disrupting chemicals",
"tipping points",
"vEmbryo",
"virtual embryo",
"virtual tissues"
]
|
| license | https://pasteur.epa.gov/license/sciencehub-license.html |
| modified | 2019-08-08 |
| programCode |
[
"020:095"
]
|
| publisher |
{
"name": "U.S. EPA Office of Research and Development (ORD)",
"subOrganizationOf": {
"name": "U.S. Environmental Protection Agency",
"subOrganizationOf": {
"name": "U.S. Government"
}
}
}
|
| references |
[
"https://doi.org/10.1093/toxsci/kfz238"
]
|
| rights |
null
|
| title | Development of an In Vitro Human Thyroid Microtissue Model for Chemical Screening |
