Human Iodotyrosine Deiodinase Inhibition Assay: Screening of ToxCast Phase 1_v2, Phase 2, and e1k Chemical Libraries
The excel spreadsheet contains the resultant data from an assay for chemical inhibition of human Iodotyrosine Deiodinase (IYD) enzyme activity screened against the ToxCast Phase 1_v2, Phase 2, and e1k chemical libraries, as well as a few additional target chemicals from ToxCast Phase 3 library or used in assay development. Over 1,800 chemicals were tested in total. This data set includes the list of chemicals tested and the median, minimum, and maximum inhibition produced by each chemical when screened at target high concentration of 200 µM. 154 chemicals were further tested in concentration response, and the median, minimum, and maximum inhibition produced at each concentration for those chemicals are included. A model inhibitor (3-Nitro-L-Tyrosine) was included on each plate as a positive control, with concentration response data for those curves also included in the data set.
This dataset is associated with the following publication:
Olker, J., J. Korte, J. Denny, J. Haselman, P. Hartig, M. Cardon, M. Hornung, and S. Degitz. In Vitro Screening for Chemical Inhibition of the Iodide Recycling Enzyme, Iodotyrosine Deiodinase. TOXICOLOGY IN VITRO. Elsevier Science Ltd, New York, NY, 71: 105073, (2021).
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"020:00"
]
|
| contactPoint |
{
"fn": "Jennifer Olker",
"hasEmail": "mailto:olker.jennifer@epa.gov"
}
|
| description | The excel spreadsheet contains the resultant data from an assay for chemical inhibition of human Iodotyrosine Deiodinase (IYD) enzyme activity screened against the ToxCast Phase 1_v2, Phase 2, and e1k chemical libraries, as well as a few additional target chemicals from ToxCast Phase 3 library or used in assay development. Over 1,800 chemicals were tested in total. This data set includes the list of chemicals tested and the median, minimum, and maximum inhibition produced by each chemical when screened at target high concentration of 200 µM. 154 chemicals were further tested in concentration response, and the median, minimum, and maximum inhibition produced at each concentration for those chemicals are included. A model inhibitor (3-Nitro-L-Tyrosine) was included on each plate as a positive control, with concentration response data for those curves also included in the data set. This dataset is associated with the following publication: Olker, J., J. Korte, J. Denny, J. Haselman, P. Hartig, M. Cardon, M. Hornung, and S. Degitz. In Vitro Screening for Chemical Inhibition of the Iodide Recycling Enzyme, Iodotyrosine Deiodinase. TOXICOLOGY IN VITRO. Elsevier Science Ltd, New York, NY, 71: 105073, (2021). |
| distribution |
[
{
"title": "Olker_et_al_IYD_in vitro screening_200610.xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1518772/Olker_et_al_IYD_in%20vitro%20screening_200610.xlsx"
}
]
|
| identifier | https://doi.org/10.23719/1518772 |
| keyword |
[
"Chemical Screening",
"In vitro assay",
"endocrine",
"iodotyrosine deiodinase",
"thyroid hormone"
]
|
| license | https://pasteur.epa.gov/license/sciencehub-license.html |
| modified | 2020-06-10 |
| programCode |
[
"020:095"
]
|
| publisher |
{
"name": "U.S. EPA Office of Research and Development (ORD)",
"subOrganizationOf": {
"name": "U.S. Environmental Protection Agency",
"subOrganizationOf": {
"name": "U.S. Government"
}
}
}
|
| references |
[
"https://doi.org/10.1016/j.tiv.2020.105073"
]
|
| rights |
null
|
| title | Human Iodotyrosine Deiodinase Inhibition Assay: Screening of ToxCast Phase 1_v2, Phase 2, and e1k Chemical Libraries |
